Shang Yue, Khafipour Ehsan, Derakhshani Hooman, Sarna Lindsei K, Woo Connie W, Siow Yaw L, O Karmin
Laboratory of Integrative Biology, CCARM, St. Boniface Hospital Research Centre, 351 Tache Avenue, Winnipeg, MB, R2H 2A6, Canada.
Department of Animal Science, Winnipeg, Canada.
Lipids. 2017 Jun;52(6):499-511. doi: 10.1007/s11745-017-4253-2. Epub 2017 Apr 20.
The gut microbiota is proposed as a "metabolic organ" involved in energy utilization and is associated with obesity. Dietary intervention is one of the approaches for obesity management. Changes in dietary components have significant impacts on host metabolism and gut microbiota. In the present study, we examined the influence of dietary fat intervention on the modification of gut mucosa-associated microbiota profile along with body weight and metabolic parameter changes. Male C57BL/6J mice (6-week old) were fed a low fat diet (10% kcal fat) as a control or a high fat diet (HFD 60% kcal fat) for 7 weeks. In another group, mice were fed HFD for 5 weeks followed by low fat control diet for 2 weeks (HFD + Control). At 7 weeks, body weight gain, blood glucose and hepatic triacylglycerol levels of mice fed a HFD were significantly higher than that of the control group and the HFD + Control group. There were significant differences in the diversity and predicted functional properties of microbiota in the cecum and colon mucosa between the control group and the HFD group. HFD feeding reduced the ratio of Bacteroidetes to Firmicutes, a microbiota pattern often associated with obesity. The HFD + Control diet partially restored the diversity and composition of microbiota in the cecum to the pattern observed in mice fed a control diet. These results suggest that short-term high fat diet withdrawal can restore metabolic changes and prevent excess body weight gain, however, long-term dietary intervention may be required to optimize the restoration of gut microbiota in mouse.
肠道微生物群被认为是参与能量利用的“代谢器官”，并与肥胖有关。饮食干预是肥胖管理的方法之一。饮食成分的变化对宿主代谢和肠道微生物群有重大影响。在本研究中，我们研究了饮食脂肪干预对肠道黏膜相关微生物群谱改变以及体重和代谢参数变化的影响。将6周龄的雄性C57BL/6J小鼠分为两组，一组喂食低脂饮食（10%千卡脂肪）作为对照，另一组喂食高脂饮食（60%千卡脂肪），持续7周。在另一组中，小鼠先喂食高脂饮食5周，然后喂食低脂对照饮食2周（高脂饮食+对照）。7周时，喂食高脂饮食的小鼠体重增加、血糖和肝脏三酰甘油水平显著高于对照组和高脂饮食+对照的小鼠。对照组和高脂饮食组之间盲肠和结肠黏膜中微生物群的多样性和预测功能特性存在显著差异。高脂饮食喂养降低了拟杆菌门与厚壁菌门的比例，这种微生物群模式通常与肥胖有关。高脂饮食+对照饮食部分恢复了盲肠中微生物群的多样性和组成，使其恢复到喂食对照饮食小鼠中观察到的模式。这些结果表明，短期停止高脂饮食可以恢复代谢变化并防止体重过度增加，然而，可能需要长期的饮食干预来优化小鼠肠道微生物群的恢复。
