Karyakin V B, Vasil'ev D S, Zhuravin I A, Zaitsev A V, Magazanik L G
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia.
St. Petersburg State University, St. Petersburg, Russia.
Dokl Biol Sci. 2017 Jan;472(1):4-7. doi: 10.1134/S0012496617010045. Epub 2017 Apr 21.
We studied early alterations in the GABAergic system of the rat hippocampus in the lithium-pilocarpine model of epilepsy. Twenty-four hours after the pilocarpine treatment, a decrease in the number of calretinin-positive interneurons was observed in the CA1 field of the hippocampus, whereas the number of parvalbumin-positive interneurons remained unchanged. The decreased levels of the GABA-synthesizing enzyme glutamic acid decarboxylase (GAD67) and the membrane GABA transporter GAT1 were revealed using Western blot analysis. These data indicate an altered excitation/inhibition balance in the hippocampus with excitation dominance.
我们在癫痫的锂-匹罗卡品模型中研究了大鼠海马体GABA能系统的早期变化。匹罗卡品治疗24小时后，在海马体CA1区观察到钙视网膜蛋白阳性中间神经元数量减少，而小白蛋白阳性中间神经元数量保持不变。使用蛋白质免疫印迹分析显示，GABA合成酶谷氨酸脱羧酶（GAD67）和膜GABA转运体GAT1的水平降低。这些数据表明海马体中兴奋/抑制平衡发生改变，以兴奋占主导。
