前驱期帕金森病胃肠道组织中α-突触核蛋白的路易病理研究

Lewy pathological study on α-synuclein in gastrointestinal tissues of prodromal Parkinson's disease.

作者信息

Lu Q-B, Zhu Z-F, Zhang H-P, Luo W-F

机构信息

Department of Neurology, the Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.

出版信息

Eur Rev Med Pharmacol Sci. 2017 Apr;21(7):1514-1521.

PMID:28429355

Abstract

OBJECTIVE

In the gastrointestinal neural system, the emergence of Lewy Body (LB) is usually earlier than the clinical diagnosis of Parkinson (PD) motor symptoms. Therefore, this study is aimed to explore whether the LB in the gastrointestinal tract of prodromal PD patients.

PATIENTS AND METHODS

98 paraffin embedded tissue specimens from 57 PD patients were collected in the Second Affiliated Hospital of Soochow University archives, as well as 98 tissue specimens of 90 non-PD patients undergone surgical resection. The pathological sections were stained by the immune-histochemistry method. The positive staining of aggregated a-Synuclein (a-a-syn) and phosphorylated a-Synuclein (p-a-syn) in gastrointestinal tract were counted to analyze the distribution of the expression of a/p-a-syn in the prodromal PD patients before diagnosis (0-5 years, 6-10 years, 11-20 years).

RESULTS

According to results from the immunohistochemical staining of a/p-α-syn, 35 (52.23%) and 30 (44.78%) tissue blocks were positively stained respectively in total 67 tissue blocks provided from 39 prodromal PD patients. And there were 46 (46.94%) and 25 (25.51%) positive staining in 98 tissue blocks from the control group. In 31 tissue blocks collected from 18 PD patients, there were 19 (61.29%) and 15 (48.39%) tissue blocks with the a/p-a-syn positive staining. Compared with non-PD patients from the control group, the a-a-syn positive rate was not significantly increased in the pre- and post-diagnosis PD patients (p>0.05). However, p-a-syn of both groups increased significantly (p<0.05). Also, the positive expression rate of a/p-a-syn in the 6-10-year group was lower than that in the 0-5-year and 11-20-year, and the positive rate of the 0-5-year group was the highest (p<0.05).

CONCLUSIONS

p-a-syn as the main component of Lewy body of nervous system in the gastrointestinal track may be used as a characteristic predictive marker of PD and the prevention of PD disease.

摘要

目的

在胃肠神经系统中，路易小体（LB）的出现通常早于帕金森病（PD）运动症状的临床诊断。因此，本研究旨在探讨前驱期PD患者胃肠道中是否存在LB。

患者与方法

收集苏州大学附属第二医院存档的57例PD患者的98份石蜡包埋组织标本，以及90例接受手术切除的非PD患者的98份组织标本。病理切片采用免疫组织化学方法染色。计数胃肠道中聚集的α-突触核蛋白（a-a-syn）和磷酸化α-突触核蛋白（p-a-syn）的阳性染色情况，以分析前驱期PD患者在诊断前（0至5年、6至10年、11至20年）a/p-a-syn表达的分布情况。

结果

根据a/p-α-syn免疫组织化学染色结果，39例前驱期PD患者提供的67个组织块中，分别有35个（52.23%）和30个（44.78%）组织块呈阳性染色。对照组98个组织块中有46个（46.94%）和25个（25.51%）呈阳性染色。在18例PD患者收集的31个组织块中，有19个（61.29%）和15个（48.39%）组织块a/p-a-syn呈阳性染色。与对照组非PD患者相比，诊断前后PD患者的a-a-syn阳性率无显著增加（p>0.05）。然而，两组的p-a-syn均显著增加（p<0.05）。此外，6至10年组a/p-a-syn的阳性表达率低于0至5年组和11至20年组，0至5年组的阳性率最高（p<0.05）。