Armstrong M A, Shah S, Hawkins S A, Bell A L, Roberts S D
Department of Microbiology and Immunobiology, Queen's University of Belfast, Northern Ireland.
Ann Neurol. 1988 Jul;24(1):12-6. doi: 10.1002/ana.410240104.
Ecto-5'nucleotidase (5'NT) activity in the plasma membrane of peripheral blood monocytes from patients with multiple sclerosis (MS), rheumatoid arthritis (RA), myasthenia gravis (MG), motor neuron disease (MND), and from normal control subjects of similar age was determined by radioisotopic assay. The activity in unstimulated monocytes cultured for 24 and 48 hours was found to be higher than normal in 56% of patients with active relapsing MS, 29% of patients with RA, and 7% of patients in the MG/MND group. While the enzyme activity was reduced after cultivation of the cells with recombinant interferon-gamma (gamma-IFN) in all of the groups studied, the percentage reduction was significantly greater in monocytes from patients with active relapsing MS who were in relapse at the time of sampling (p = 0.03). HLA-DR expression was monitored using immunofluorescence staining with monoclonal antibody and showed that similar numbers of monocytes in all patient and control groups expressed this antigen. Thus, while high monocyte 5'NT activity was found principally in patients with active relapsing MS and in some patients with RA, the monocytes from patients with MS were, in addition, exquisitely sensitive to stimulation with gamma-IFN.
采用放射性同位素分析法测定了多发性硬化症（MS）、类风湿性关节炎（RA）、重症肌无力（MG）、运动神经元病（MND）患者以及年龄相仿的正常对照者外周血单核细胞膜上的ecto-5'核苷酸酶（5'NT）活性。发现在培养24小时和48小时的未受刺激单核细胞中，56%处于复发活动期的MS患者、29%的RA患者以及7%的MG/MND组患者的酶活性高于正常水平。虽然在所研究的所有组中，用重组干扰素-γ（γ-IFN）培养细胞后酶活性均降低，但在采样时处于复发期的复发活动期MS患者的单核细胞中，酶活性降低的百分比显著更大（p = 0.03）。使用单克隆抗体通过免疫荧光染色监测HLA-DR表达，结果显示所有患者组和对照组中表达该抗原的单核细胞数量相似。因此，虽然单核细胞5'NT活性升高主要见于复发活动期MS患者和部分RA患者，但MS患者的单核细胞对γ-IFN刺激格外敏感。
