Miyauchi T, Sano Y, Hiroshima O, Yuzuriha T, Sugishita Y, Ishikawa T, Saito A, Goto K
Department of Internal Medicine, University of Tsukuba, Japan.
Biochem Biophys Res Commun. 1988 Aug 30;155(1):289-94. doi: 10.1016/s0006-291x(88)81082-9.
Calcitonin gene-related peptide (CGRP) in porcine ventricular muscles. positive inotropic effects in the isolated, electrically driven false tendon of the porcine heart. Specific CGRP-binding sites were present in solubilized membrane fractions; the dissociation constant (Kd) and the maximum binding (Bmax) were 50.4 pM and 180 fmol/mg protein, respectively. SDS-PAGE analysis of CGRP-binding sites revealed the molecular mass of 70 K and 120 K. Few CGRP-like immunoreactive nerves were present in the ventricular muscle layer. These results indicate that CGRP activates specific receptor sites on the ventricular muscles and causes positive inotropic responses. CGRP receptors in ventricles are likely to be activated by circulating CGRP.
猪心室肌中的降钙素基因相关肽（CGRP）。对猪心脏分离的、电驱动的假腱具有正性肌力作用。在可溶膜组分中存在特异性CGRP结合位点；解离常数（Kd）和最大结合量（Bmax）分别为50.4 pM和180 fmol/mg蛋白质。CGRP结合位点的SDS-PAGE分析显示分子量为70K和120K。心室肌层中存在少量CGRP样免疫反应性神经。这些结果表明CGRP激活心室肌上的特异性受体位点并引起正性肌力反应。心室中的CGRP受体可能被循环中的CGRP激活。
