Hu Long-Wei, Wang Xiao, Jiang Xin-Qun, Xu Li-Qun, Pan Hong-Ya
Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, PR China; Oral Bioengineering Laboratory/Regenerative Medicine Laboratory, Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, PR China.
Oral Bioengineering Laboratory/Regenerative Medicine Laboratory, Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, PR China.
Exp Cell Res. 2017 Aug 1;357(1):25-32. doi: 10.1016/j.yexcr.2017.04.018. Epub 2017 Apr 19.
Bone marrow-derived mesenchymal stem cells (BMSCs) are a major source of osteoblasts and are crucial for bone remolding and repair and thus they are widely used for tissue engineering applications. Tissue engineering in combination with gene therapy is considered as a promising approach in new bone regeneration. Endothelin-1（EDN-1）is produced by vascular endothelial cells which plays an important role during bone development. However, its role in BMSCs remains largely unknown. We established EDN-1 overexpressed BMSCs, proliferation ability and osteogenesis differentiation were detected respectively. Transduced BMSCs were then combined with CPC-scaffold to repair calvarial defects in rats to evaluate the in-vivo osteogenic potential of EDN-1. EDN-1 overexpressed BMSCs showed increased proliferation and significantly increased osteogenesis potential ability than vector transfected control. The in-vivo data also revealed more new bone formation with higher bone mineral density and number of trabeculae in EDN-1 overexpressed BMSCs. These findings have demonstrated the influence of EDN-1 on differentiation potential of BMSCs, which suggest that EDN-1 may be a new promising agent for bone tissue engineering.
骨髓间充质干细胞（BMSCs）是成骨细胞的主要来源，对骨重塑和修复至关重要，因此被广泛应用于组织工程领域。组织工程与基因治疗相结合被认为是促进新骨再生的一种有前景的方法。内皮素-1（EDN-1）由血管内皮细胞产生，在骨骼发育过程中发挥重要作用。然而，其在BMSCs中的作用仍 largely未知。我们建立了EDN-1过表达的BMSCs，并分别检测其增殖能力和成骨分化能力。然后将转导的BMSCs与磷酸钙骨水泥支架结合，修复大鼠颅骨缺损，以评估EDN-1的体内成骨潜力。EDN-1过表达的BMSCs显示出增殖增加，并且与载体转染对照相比，成骨潜力能力显著增加。体内数据还显示，EDN-1过表达的BMSCs中有更多的新骨形成，骨矿物质密度更高，小梁数量更多。这些发现证明了EDN-1对BMSCs分化潜力的影响，这表明EDN-1可能是骨组织工程中一种新的有前景的药物。
