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一种用于阿魏酸酯酶初步筛选和特性鉴定的酶谱技术。

A Zymogram technique for preliminary screening and characterization of feruloyl esterases.

作者信息

Palaniswamy Sakthi Kumaran, Govindaswamy Vijayalakshmi

机构信息

AcSIR - Academy of Scientific & Innovative Research, New Delhi, India; Department of Microbiology & Fermentation Technology, CSIR-CFTRI, Mysore, India, India.

AcSIR - Academy of Scientific & Innovative Research, New Delhi, India; Department of Microbiology & Fermentation Technology, CSIR-CFTRI, Mysore, India, India.

出版信息

Enzyme Microb Technol. 2017 Jun;101:57-59. doi: 10.1016/j.enzmictec.2017.03.006. Epub 2017 Mar 16.

Abstract

We report here, for the first time, a zymogram technique designed for rapid screening of feruloyl esterase using ethyl ferulate as enzyme substrate and casein precipitation as enzyme activity indicator.

摘要

我们在此首次报道了一种酶谱技术,该技术以阿魏酸乙酯作为酶底物,酪蛋白沉淀作为酶活性指示剂,用于快速筛选阿魏酸酯酶。

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