Thiara A S, Cundliffe E
Department of Biochemistry, University of Leicester, UK.
EMBO J. 1988 Jul;7(7):2255-9. doi: 10.1002/j.1460-2075.1988.tb03065.x.
A gyrB gene from Streptomyces sphaeroides, a producer of novobiocin, has been cloned in Streptomyces lividans, where it conferred resistance to novobiocin. The Streptomyces gyrB gene was sufficiently similar to a Bacillus subtilis gyrB probe to be specifically recognized during Southern analysis. Partial purification of DNA gyrase by affinity chromatography revealed the presence of two such activities (differing in their responses to novobiocin) in the clone. The product of the cloned gene, a novobiocin-resistant DNA gyrase B subunit, was identified in vitro by coupled transcription--translation as a 79-kd protein.
新霉素产生菌球形链霉菌（Streptomyces sphaeroides）的gyrB基因已克隆到淡紫链霉菌（Streptomyces lividans）中，该基因赋予了淡紫链霉菌对新霉素的抗性。链霉菌gyrB基因与枯草芽孢杆菌（Bacillus subtilis）的gyrB探针足够相似，能够在Southern分析中被特异性识别。通过亲和层析对DNA促旋酶进行部分纯化，结果显示该克隆中存在两种这样的活性（对新霉素的反应不同）。通过体外偶联转录-翻译，将克隆基因的产物鉴定为一种79-kd的蛋白，即一种对新霉素具有抗性的DNA促旋酶B亚基。
Zotero 插件
