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肺炎链球菌DNA促旋酶B亚基中丝氨酸127到亮氨酸的替换与新生霉素耐药性有关。

Ser-127-to-Leu substitution in the DNA gyrase B subunit of Streptococcus pneumoniae is implicated in novobiocin resistance.

作者信息

Muñoz R, Bustamante M, de la Campa A G

机构信息

Unidad de Genética Bacteriana, Instituto de Salud Carlos III, Majadahonda, Spain.

出版信息

J Bacteriol. 1995 Jul;177(14):4166-70. doi: 10.1128/jb.177.14.4166-4170.1995.

DOI:10.1128/jb.177.14.4166-4170.1995
PMID:7608096
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC177155/
Abstract

We report the cloning of the gyrB gene from Streptococcus pneumoniae 533 that carries the nov-1 allele. The gyrB gene codes for a protein homologous to the gyrase B subunit of archaebacteria and eubacteria. The same amino acid substitution (Ser-127 to Leu) confers novobiocin resistance on four isolates of S. pneumoniae. This amino acid position is equivalent to Val-120 of Escherichia coli GyrB, a residue that lies inside the ATP-binding domain as revealed by the crystal structure of the protein.

摘要

我们报道了从携带nov-1等位基因的肺炎链球菌533中克隆gyrB基因。gyrB基因编码一种与古细菌和真细菌的gyrase B亚基同源的蛋白质。相同的氨基酸取代（丝氨酸127突变为亮氨酸）赋予了四株肺炎链球菌新霉素抗性。该氨基酸位置等同于大肠杆菌GyrB的缬氨酸120，蛋白质晶体结构显示该残基位于ATP结合域内。

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J Bacteriol. 1995 Jul;177(14):4166-70. doi: 10.1128/jb.177.14.4166-4170.1995.
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本文引用的文献

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Expression and analysis of two gyrB genes from the novobiocin producer, Streptomyces sphaeroides.来自新生霉素产生菌球形链霉菌的两个gyrB基因的表达与分析。
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