Zhou Yang, Wu Bo, Li Jiang-Hua, Nan Gang, Jiang Jian-Li, Chen Zhi-Nan
National Translational Science Center for Molecular Medicine, Cell Engineering Research Centre and Department of Cell Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, 169 Changle West Road, Xi'an 710032, PR China.
Exp Cell Res. 2017 Aug 1;357(1):9-16. doi: 10.1016/j.yexcr.2017.04.020. Epub 2017 Apr 19.
Rab22a is a member of the Ras-related small GTPase family, which plays a key role in regulating the recycling of cargo proteins entering cells through clathrin-independent endocytosis (CIE). Rab22a is overexpressed in different cancer types, including liver cancer, malignant melanoma, ovarian cancer and osteosarcoma. However, its oncogenic role remains unknown. In this study, we found that silencing of Rab22a suppressed the migration and invasion of lung cancer cells. Furthermore, Rab22a interacts with CD147, and knockdown of Rab22a blocks CD147 recycling and promotes CD147 degradation. Taken together, our findings indicate that Rab22a enhances recycling of CD147, which is required for lung cancer cell migration and invasion,and targeting CD147 recycling may be a rational strategy for lung cancer therapy.
Rab22a是Ras相关小GTP酶家族的成员，在调节通过网格蛋白非依赖型内吞作用（CIE）进入细胞的货物蛋白的循环利用中起关键作用。Rab22a在包括肝癌、恶性黑色素瘤、卵巢癌和骨肉瘤在内的不同癌症类型中过表达。然而，其致癌作用尚不清楚。在本研究中，我们发现沉默Rab22a可抑制肺癌细胞的迁移和侵袭。此外，Rab22a与CD147相互作用，敲低Rab22a可阻断CD147的循环利用并促进CD147的降解。综上所述，我们的研究结果表明，Rab22a增强了CD147的循环利用，而这是肺癌细胞迁移和侵袭所必需的，靶向CD147的循环利用可能是肺癌治疗的合理策略。
