Xia Xichao, Xue Shipeng, Wang Xiying, Zhang Qingyuan, Huang Chuanfeng, Guo Lianghong, Yao Lunguang
Medical College of Pingdingshan University, Pingdingshan, 467000, Henan Province, China; State Key Laboratory of Environmental Chemistry and Eco-toxicology, Research Centre for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; Department of Basic Medicine, Nanyang Medical College, Nanyang, 473061, Henan Province, China.
Department of Basic Medicine, Nanyang Medical College, Nanyang, 473061, Henan Province, China.
Fish Shellfish Immunol. 2017 Jun;65:213-225. doi: 10.1016/j.fsi.2017.04.017. Epub 2017 Apr 19.
Heat shock proteins (HSPs) play an important role in adaption of environmental stress by protein folding, membrane translocation, degradation of misfolded proteins and other regulatory processes. Our previous study showed oxidative stress generated from polybrominated diphenyl ether-47 (PBDE-47) could cause an acute toxicity on freshwater bivalve Anodonta Woodiana, but the effect of chronic toxicity need to be elucidated. In order to further investigate the chronic effect of PBDE-47, clams A. Woodiana were randomly divided into the PBDE-47 treated group administrated with PBDE-47 at a concentration 3.36 μg/L and control group treated with a similar volume dimethyl sulfoxide. Two complete HSP sequences were isolated from A. Woodianaa and respectively named AwHSP60 and AwHSP70. They were widely distributed in foot, gill, hepatopancreas, adductor muscle, heart, hemocytes and mantle. Administration of PBDE-47 could result in a significant up-regulation of AwHSP60 and AwHSP70 expressions in the hepatopancreas, gill and hemocytes. In the hepatopancreas, compared with that of control group, mRNA level of AwHSP60 increased more than 89.9% (P < 0.05) from day 1-15, AwHSP70 increased more 2.79 times (P < 0.01). In the gill, during experiment observed, expression of AwHSP60 increased more 2.09 times (P < 0.01) in contrasted with that of control group. Significant up-regulation of AwHSP70 expression showed a reversed U shape. In the hemocytes, AwHSP60 and AwHSP70 expressions of PBDE-47 treated group respectively increased more 2.09 times (P < 0.05) and 1.81 times (P < 0.05) compared with that of control group. These results indicated that up-regulations of AwHSP60 and AwHSP70 expression are contribute to enhancing adaption of bivalve A. Woodiana exposed to PBDE-47 treatment.
热休克蛋白（HSPs）通过蛋白质折叠、膜转运、错误折叠蛋白质的降解及其他调节过程，在环境应激适应中发挥重要作用。我们之前的研究表明，多溴二苯醚-47（PBDE-47）产生的氧化应激可对淡水双壳贝类背角无齿蚌造成急性毒性，但慢性毒性效应尚需阐明。为进一步研究PBDE-47的慢性效应，将背角无齿蚌随机分为PBDE-47处理组（以3.36μg/L的浓度给予PBDE-47）和对照组（给予等体积的二甲基亚砜）。从背角无齿蚌中分离出两个完整的HSP序列，分别命名为AwHSP60和AwHSP70。它们广泛分布于足部、鳃、肝胰腺、闭壳肌、心脏、血细胞和外套膜。给予PBDE-47可导致肝胰腺、鳃和血细胞中AwHSP60和AwHSP70表达显著上调。在肝胰腺中，与对照组相比，AwHSP60的mRNA水平在第1至15天增加超过89.9%（P<0.05），AwHSP70增加2.79倍（P<0.01）。在鳃中，在实验观察期间，AwHSP60的表达与对照组相比增加了2.09倍（P<0.01）。AwHSP70表达的显著上调呈倒U形。在血细胞中，PBDE-47处理组的AwHSP60和AwHSP70表达与对照组相比分别增加了2.09倍（P<0.05）和1.81倍（P<0.05）。这些结果表明，AwHSP60和AwHSP70表达的上调有助于增强背角无齿蚌对PBDE-47处理的适应性。
