Baskic Dejan, Vukovic Vuk R, Popovic Suzana, Djurdjevic Predrag, Zaric Milan, Nikolic Ivana, Zelen Ivanka, Mitrovic Marina, Avramovic Dusko, Mijailovic Zeljko
Department of Microbiology and Immunology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia; Public Health Institute, Nikole Pasica 1, 34000 Kragujevac, Serbia.
Garrison Clinic, Milovana Gusica 91, 34000 Kragujevac, Serbia.
Cytokine. 2017 Aug;96:185-188. doi: 10.1016/j.cyto.2017.04.008. Epub 2017 Apr 21.
The data addressing cytokine profile in chronically infected HCV patients are conflicting, ranging from Th1 or Th2 cytokine prevalence to the expression of both types of cytokines. Therefore, the aim of this study was to evaluate cytokine profile in these patients. Cytokine sera levels in HCV patients and healthy controls were evaluated using 13plex FlowCytomix Multiplex. Median values of both proinflammatory and anti-inflammatory cytokines were lower in HCV patients then in controls. In addition, the number of subjects producing detectable quantities of cytokines was significantly lower in the group of HCV patients. Yet, cytokine levels in those patients were remarkably heterogeneous ranging from low to extremely high, much higher than the maximal values in control group. Similarly, grouping data according to HCV genotype, HCV RNA load, ALT/AST ratio and the stage of fibrosis showed marked standard deviations, reflecting high intragroup diversity. No correlation was found between each disease-related factor and cytokine levels. Patients investigated in our and similar studies were disparate pursuant to characteristics of the hosts, pathogen and course of the disease. Therefore, the inconsistency of the literature data regarding cytokine pattern in chronic HCV patients may be a consequence of the disregarded/overlooked heterogeneity of these patients.
关于慢性丙型肝炎病毒（HCV）感染患者细胞因子谱的数据存在矛盾，从Th1或Th2细胞因子的流行情况到两种细胞因子的表达均有不同报道。因此，本研究的目的是评估这些患者的细胞因子谱。使用13联流式细胞仪多重检测法评估HCV患者和健康对照者血清中的细胞因子水平。HCV患者中促炎和抗炎细胞因子的中位数均低于对照组。此外，HCV患者组中产生可检测量细胞因子的受试者数量明显较少。然而，这些患者的细胞因子水平差异极大，从低到极高，远高于对照组的最大值。同样，根据HCV基因型、HCV RNA载量、谷丙转氨酶/谷草转氨酶比值和纤维化阶段对数据进行分组时，显示出明显的标准差，反映出组内高度的多样性。未发现各疾病相关因素与细胞因子水平之间存在相关性。在我们的研究以及类似研究中所调查的患者，在宿主、病原体和疾病进程的特征方面存在差异。因此，关于慢性HCV患者细胞因子模式的文献数据不一致，可能是由于这些患者的异质性被忽视/忽略所致。
