Enzyme Biotechnology & Genetic Engineering Group, University of Ioannina, Department of Chemistry, Ioannina 45110, Greece.
Food Biotechnology Group, University of Patras, Department of Chemistry, Patras 26500, Greece.
Bioresour Technol. 2017 Aug;238:122-128. doi: 10.1016/j.biortech.2017.04.028. Epub 2017 Apr 11.
In this work we suggest a methodology comprising the design and use of cost-effective, sustainable, and environmentally friendly process for biofuel production compatible with the market demands. A new generation biofuel is produced using fatty acids, which were generated from acidogenesis of industrial wastes of bioethanol distilleries, and esterified with selected alcohols by immobilized Candida antarctica Lipase-B. Suitable reactors with significant parameters and conditions were studied through experimental design, and novel esterification processes were suggested; among others, the continuous removal of the produced water was provided. Finally, economically sustainable biofuel production was achieved providing high ester yield (<97%) along with augmented concentration (3.35M) in the reaction mixtures at relatively short esterification times, whereas the immobilized lipase maintained over 90% of its initial esterifying ability after reused for ten cycles.
在这项工作中，我们提出了一种方法学，包括设计和使用具有成本效益、可持续和环保的生物燃料生产工艺，以满足市场需求。使用脂肪酸生产新一代生物燃料，这些脂肪酸是通过生物乙醇蒸馏厂工业废物的酸发酵产生的，并通过固定化南极假丝酵母脂肪酶-B 与选定的醇酯化。通过实验设计研究了具有显著参数和条件的合适反应器，并提出了新型酯化工艺；其中包括连续去除产生的水。最后，实现了经济可持续的生物燃料生产，提供了高酯收率（<97%），同时在相对较短的酯化时间内提高了反应混合物中的浓度（3.35M），而固定化脂肪酶在重复使用十次后仍保持超过 90%的初始酯化能力。
