用蛋白A酶联免疫吸附试验检测小鼠和豚鼠血清中的仙台病毒抗体。

Detection of Sendai virus antibody in mouse and guinea pig sera by an enzyme-linked immunosorbent assay with protein A.

作者信息

Takakura A, Kagiyama N, Sakurai Y, Suzuki H, Kobayashi N, Terada E

机构信息

Central Institute for Experimental Animals, Kawasaki, Japan.

出版信息

Jikken Dobutsu. 1988 Jul;37(3):279-83. doi: 10.1538/expanim1978.37.3_279.

DOI:10.1538/expanim1978.37.3_279

PMID:2843392

Abstract

An enzyme-linked immunosorbent assay using horseradish peroxidase (HRPO)-labeled protein A (P-ELISA) was established for detection of Sendai virus (SV) antibody in mouse and guinea pig sera. Sensitivity and specificity of P-ELISA were compared with those of ordinary ELISA using HRPO-labeled immunoglobulin G (IgG-ELISA) and the hemagglutination inhibition (HI) test. P-ELISA was 100 to 1,000 times more sensitive than the HI test for detection of the antibody in SV-naturally infected mice. P-ELISA and IgG-ELISA showed similar sensitivities for detection of the antibody in naturally infected mouse and guinea pig sera. A high specificity was demonstrated in P-ELISA with a cut-off optical density value of 0.2 (492 nm), while a non-specific reaction was observed when IgG-ELISA was used to both mouse and guinea pig sera at a low dilution (1:10-20). The antibody in rat sera was not detected by P-ELISA although it was realized by IgG-ELISA.

摘要

建立了一种使用辣根过氧化物酶（HRPO）标记的蛋白A的酶联免疫吸附测定法（P-ELISA），用于检测小鼠和豚鼠血清中的仙台病毒（SV）抗体。将P-ELISA的敏感性和特异性与使用HRPO标记的免疫球蛋白G的普通ELISA（IgG-ELISA）以及血凝抑制（HI）试验进行了比较。在检测自然感染仙台病毒的小鼠抗体方面，P-ELISA比HI试验敏感100至1000倍。在检测自然感染的小鼠和豚鼠血清中的抗体时，P-ELISA和IgG-ELISA显示出相似的敏感性。当P-ELISA的截止光密度值为0.2（492nm）时，显示出高特异性，而当IgG-ELISA用于低稀释度（1:10 - 20）的小鼠和豚鼠血清时，观察到非特异性反应。尽管IgG-ELISA能检测到大鼠血清中的抗体，但P-ELISA未检测到。