Kabir A M, Aiba Y, Takagi A, Kamiya S, Miwa T, Koga Y
Department of Infectious Diseases, Tokai University, School of Medicine, Kanagawa, Japan.
Gut. 1997 Jul;41(1):49-55. doi: 10.1136/gut.41.1.49.
Helicobacter pylori is a bacterium which causes gastric inflammatory diseases. Oral inoculation of H pylori usually results in only a temporary colonisation without a successful infection in the stomach of conventional mice in which lactobacilli are the predominant indigenous bacteria.
To determine whether lactobacilli exert an inhibitory effect on colonisation by H pylori in the stomach.
The effects of H pylori on attachment to murine and human gastric epithelial cells and the H pylori mediated release of interleukin-8 (IL-8) by these cells were examined in vitro. Lactobacillus salivarius infected gnotobiotic BALB/c mice and control germ free mice were inoculated orally with H pylori to examine whether L salivarius can inhibit colonisation by H pylori.
L salivarius inhibited both the attachment and IL-8 release in vitro. H pylori could not colonise the stomach of L salivarius infected gnotobiotic BALB/c mice, but colonised in large numbers and subsequently caused active gastritis in germ free mice. In addition, L salivarius given after H pylori implantation could eliminate colonisation by H pylori.
These findings suggest the possibility of lactobacilli being used as probiotic agents against H pylori.
幽门螺杆菌是一种可引发胃部炎症性疾病的细菌。在以乳酸菌为主要本土细菌的传统小鼠胃中，口服接种幽门螺杆菌通常仅导致短暂定植，而无法成功感染。
确定乳酸菌是否对幽门螺杆菌在胃中的定植发挥抑制作用。
在体外检测幽门螺杆菌对鼠类和人类胃上皮细胞的黏附作用以及这些细胞由幽门螺杆菌介导的白细胞介素-8（IL-8）释放情况。用唾液乳杆菌感染无菌BALB/c小鼠，对感染唾液乳杆菌的无菌BALB/c小鼠和对照无菌小鼠口服接种幽门螺杆菌，以检测唾液乳杆菌是否能抑制幽门螺杆菌的定植。
唾液乳杆菌在体外抑制黏附和IL-8释放。幽门螺杆菌无法在感染唾液乳杆菌的无菌BALB/c小鼠胃中定植，但在无菌小鼠中大量定植并随后引发活动性胃炎。此外，在接种幽门螺杆菌后给予唾液乳杆菌可消除幽门螺杆菌的定植。
这些发现提示乳酸菌有可能用作抗幽门螺杆菌的益生菌制剂。
