一种具有完整编码序列的大鼠肝脏细胞色素c氧化酶亚基VIc的加工假基因。
A processed pseudogene with an intact coding sequence for rat liver cytochrome c oxidase subunit VIc.
作者信息
Seelan R S, Padmanaban G
机构信息
Department of Biochemistry, Indian Institute of Science, Bangalore.
出版信息
Gene. 1988 Jul 15;67(1):125-30. doi: 10.1016/0378-1119(88)90015-7.
The biogenesis of eukaryotic cytochrome c oxidase involves the coordinate expression of nuclear and mitochondrial genes. Very little information is available on the gene structure of nuclear-coded cytochrome c oxidase subunits in mammalian systems. We report here the isolation and complete nucleotide sequence determination of a processed pseudogene for cytochrome c oxidase subunit VIc from rat liver. The pseudogene lacks introns and the coding region is intact with no deleterious lesions; however, there are 7 amino acid (aa) differences when compared to the sequence derived from cDNA clones. The pseudogene has the potential to code for a protein of 76 aa, containing a putative 3 aa N-terminal presequence when compared to the mature bovine heart VIc subunit. Potential regulatory regions, including a TATA box, are present in the 5'-flanking region.
真核细胞细胞色素c氧化酶的生物合成涉及核基因和线粒体基因的协调表达。在哺乳动物系统中，关于核编码的细胞色素c氧化酶亚基的基因结构的信息非常少。我们在此报告从大鼠肝脏中分离出细胞色素c氧化酶亚基VIc的一个加工假基因，并测定其完整的核苷酸序列。该假基因没有内含子，编码区完整且无有害损伤；然而，与从cDNA克隆获得的序列相比，有7个氨基酸差异。与成熟的牛心VIc亚基相比，该假基因有潜力编码一个76个氨基酸的蛋白质，包含一个推定的3个氨基酸的N端前序列。潜在的调控区域，包括一个TATA盒，存在于5'侧翼区域。
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