Volonté C, Parries G S, Racker E
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853.
J Neurochem. 1988 Oct;51(4):1156-62. doi: 10.1111/j.1471-4159.1988.tb03081.x.
The effects of bradykinin (BK) and lithium on the phosphatidylinositol cycle were examined in PC12 cells cultured for 20 h in the presence [PC12(+)] or in the absence [PC12(-)] of nerve growth factor (NGF). BK (1 microM) induced a small stimulation of the incorporation of myo-[2-3H]inositol into the lipids of PC12(-) cells and a three- to fourfold stimulation of such incorporation into the lipids of PC12 (+) cells. About 15 h of incubation with NGF and greater than 10 min of incubation with BK were needed for maximal stimulation of inositol incorporation by BK. In the presence of 25 mM LiCl, BK stimulated the inositol monophosphate levels nine-fold in PC12 (-) and 30-fold in PC12 (+) cells. After incubation for 20 h with NGF, an increased binding of [3H]BK to the PC12 (+) cells was observed at 4 degrees C. Exposure of the cells for 30 min to 25 mM LiCl enhanced the effect of BK on the inositol incorporation into total inositol lipids, especially in PC12(+) cells. In these cells, LiCl in the presence of BK also increased several-fold the intracellular levels of inositol bisphosphate and inositol trisphosphate.
在存在[PC12(+)]或不存在[PC12(-)]神经生长因子(NGF)的情况下培养20小时的PC12细胞中，研究了缓激肽(BK)和锂对磷脂酰肌醇循环的影响。BK(1 microM)对PC12(-)细胞中肌醇-[2-3H]掺入脂质有轻微刺激作用，而对PC12(+)细胞中该掺入有三到四倍的刺激作用。BK对肌醇掺入的最大刺激需要用NGF孵育约15小时以及用BK孵育超过10分钟。在25 mM LiCl存在下，BK使PC12(-)细胞中的肌醇单磷酸水平增加9倍，使PC12(+)细胞中的肌醇单磷酸水平增加30倍。用NGF孵育20小时后，在4℃观察到[3H]BK与PC12(+)细胞的结合增加。将细胞暴露于25 mM LiCl 30分钟增强了BK对肌醇掺入总肌醇脂质的作用，特别是在PC12(+)细胞中。在这些细胞中，BK存在下的LiCl还使细胞内肌醇二磷酸和肌醇三磷酸水平增加了几倍。
