Janz Viktor, Trampuz Andrej, Perka Carsten F, Wassilew Georgi I
Technol Health Care. 2017 Aug 9;25(4):635-640. doi: 10.3233/THC-160660.
The aim of this study was to investigate if the cultivation of sonicate fluid (SFC) in blood culture bottles (BCB) leads to a higher rate of bacterial isolations than agar plate culture (APC) and to investigate whether the utilization of BCB leads to a reduction in culture time.
We performed a retrospective analysis of 206 revision total knee and total hip arthroplasty patients comparing the results of both synovial fluid culture and SFC in both BCB and conventional APC.
The use of BCB improved both the rate of positive bacterial isolations and reduced the culture time for synovial fluid as well as SFC. Fifty-one patients showed a bacterial isolation in SFC-APC and 101 in SFC-BCB. For synovial fluid 24 patients showed a bacterial isolation on APC and 37 showed a bacterial isolation in BCB. The synovial fluid cultures showed growth on APC after an average of 2.8 days vs. 1.8 days in BCB. The SFC-APC showed growth after an average of 4.2 days vs. 2.9 days for SFC-BCB.
The culture of synovial and sonicate fluid in BCB leads to more positive bacterial isolations and quicker bacterial growth than conventional agar plate cultures.
本研究旨在调查血液培养瓶（BCB）中超声处理液（SFC）培养是否比琼脂平板培养（APC）能获得更高的细菌分离率，并研究使用BCB是否能缩短培养时间。
我们对206例全膝关节置换术和全髋关节置换术翻修患者进行了回顾性分析，比较了BCB和传统APC中滑膜液培养及SFC的结果。
使用BCB提高了细菌阳性分离率，同时缩短了滑膜液及SFC的培养时间。51例患者在SFC-APC中出现细菌分离，101例在SFC-BCB中出现细菌分离。对于滑膜液，24例患者在APC上出现细菌分离，37例在BCB中出现细菌分离。滑膜液培养在APC上平均2.8天后生长，而在BCB中为1.8天。SFC-APC平均4.2天后生长，而SFC-BCB为2.9天。
与传统琼脂平板培养相比，BCB中滑膜液和超声处理液培养能获得更多的细菌阳性分离且细菌生长更快。
