Guo Yuntao, Chen Liwen, Sun Chengyi, Yu Chao
Department of Hepatobiliary Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, 550004 Guizhou, China.
Biomed Pharmacother. 2017 Jul;91:13-20. doi: 10.1016/j.biopha.2017.04.018. Epub 2017 Apr 22.
Increased expression of microRNA-500a (miR-500a) has been reported in the serum of hepatocellular carcinoma (HCC) patients. However, the biological effects and mechanisms of miR-500a in hepatoma cells remain unclear. In this study, we found that miR-500a expression was up-regulated in HCC cell lines and tissues, and that high levels of miR-500a was associated with poor prognosis. We found that miR-500a upregulation promoted migration and invasion in two hepatoma cell lines, HCCLM3 and SMMC7721, while miR-500a downregulation had the opposite effect. We demonstrated that miR-500a activates the Wnt/β-catenin signaling pathway by directly binding to the 3'-untranslated region (UTR) of SFRP2 and GSK-3β mRNA. In conclusion, our results indicate miR-500a promotes HCC migration and invasion through activating Wnt/β-catenin signaling by directly binding to SFPR2 and GSK-3β.
据报道，肝细胞癌（HCC）患者血清中微小RNA-500a（miR-500a）表达增加。然而，miR-500a在肝癌细胞中的生物学效应和机制仍不清楚。在本研究中，我们发现miR-500a在肝癌细胞系和组织中表达上调，且高水平的miR-500a与预后不良相关。我们发现miR-500a上调促进了两种肝癌细胞系HCCLM3和SMMC7721的迁移和侵袭，而miR-500a下调则产生相反的效果。我们证明miR-500a通过直接结合SFRP2和GSK-3β mRNA的3'-非翻译区（UTR）激活Wnt/β-连环蛋白信号通路。总之，我们的结果表明miR-500a通过直接结合SFPR2和GSK-3β激活Wnt/β-连环蛋白信号通路，促进肝癌的迁移和侵袭。
