Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, PR China.
Central laboratory, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, PR China.
J Dent. 2017 Jun;61:21-27. doi: 10.1016/j.jdent.2017.04.006. Epub 2017 Apr 22.
To investigate differentially expressed salivary peptides in the development of early childhood caries (ECC) in 3-4 year-old children.
Eighty-two caries-free children at baseline were followed-up for 1year, during which period 15 of them had developed ECC (Group C), whilst another 15 cases out of the 31 individuals who remained healthy were marked as Group H. Stimulated whole saliva samples were collected at 0, 6 and 12 months, and analyzed using weak cation exchange magnetic beads combined with matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Corresponding peptide mass fingerprints were obtained to develop a discriminating model for ECC development. Q-Exactive mass spectrometry was then performed to identify the possible proteins where these peptides might derive from.
Nine peptide peaks were found to be significantly different in Group C among the three sampling time points and might correlate with development of caries. Levels of three of them increased over time, whilst that of the other six decreased gradually. We chose three peptides (1346.6, 2603.5 and 3192.8Da) which exhibited the best capability of classification, to establish a model for children at high risk of caries. One peptide (1346.6Da) was identified to be salivary histatin-rich peptide.
Our results indicate that peptidomic methods can be applied to help identify new candidate biomarkers for the occurrence and development of ECC.
The change of salivary peptides may be an indicator of ECC, facilitating more effective measures to be taken in prevention of this disease.
探讨儿童早期龋（ECC）发展过程中唾液肽的差异表达。
82 名基线无龋的 3-4 岁儿童进行了为期 1 年的随访，其中 15 名儿童发生了 ECC（C 组），而其余 31 名保持健康的儿童中有 15 名被标记为 H 组。分别于 0、6 和 12 个月时采集刺激全唾液样本，使用弱阳离子交换磁珠联合基质辅助激光解吸/电离飞行时间质谱进行分析。获得相应的肽质量指纹图谱，建立 ECC 发展的鉴别模型。然后使用 Q-Exactive 质谱鉴定这些肽可能来自的可能蛋白质。
在三个采样时间点中，C 组有 9 个肽峰差异显著，可能与龋病的发生有关。其中 3 个的水平随时间增加而增加，而另外 6 个则逐渐减少。我们选择了 3 个具有最佳分类能力的肽（1346.6、2603.5 和 3192.8Da），建立了儿童高龋风险模型。其中一个肽（1346.6Da）被鉴定为唾液富含组蛋白的肽。
我们的研究结果表明，肽组学方法可用于帮助识别 ECC 发生和发展的新候选生物标志物。
唾液肽的变化可能是 ECC 的一个指标，有助于采取更有效的措施预防这种疾病。
