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采用DNA杂交方法对中国沿海黑腹果蝇中P因子的分布进行了研究。

The distribution of P elements in D. melanogaster along the coast of China studied by DNA hybridization method.

作者信息

Lai F, Zhao S Y, Jiang Z L, Liu Z D, Sun J W

机构信息

Institute of Genetics, Fudan University, Shanghai.

出版信息

Sci Sin B. 1988 May;31(5):563-71.

PMID:2843983
Abstract

The distribution of the P-M system in four local Drosophila melanogaster populations obtained in southern China has been examined by mating tests. There was no P strain. A HindIII fragment of the P element has been cloned, named as pPH 0.86, and used as probe in this study. The distribution of the P element itself in thirteen local D. melanogaster populations along the eastern coast of China has been studied by the method of DNA hybridization. It is found that all the populations obtained from the north of Shanghai carry P elements but few populations from the south of Shanghai carry P elements. In comparison with the mating test, the method of DNA hybridization is more direct and reliable. The origin and trends of distribution of P elements in China are discussed in this paper.

摘要

通过交配试验研究了采自中国南方的四个当地黑腹果蝇种群中P-M系统的分布情况。没有发现P品系。P元件的一个HindIII片段已被克隆,命名为pPH 0.86,并在本研究中用作探针。采用DNA杂交方法研究了P元件在中国东部沿海13个当地黑腹果蝇种群中的分布情况。发现来自上海以北的所有种群都携带P元件,而来自上海以南的种群中携带P元件的很少。与交配试验相比,DNA杂交方法更直接、更可靠。本文讨论了P元件在中国的起源和分布趋势。

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