一种有缺陷的单纯疱疹病毒1型载体在培养的外周神经元中表达大肠杆菌β-半乳糖苷酶。
A defective HSV-1 vector expresses Escherichia coli beta-galactosidase in cultured peripheral neurons.
作者信息
Geller A I, Breakefield X O
机构信息
Department of Neurology, Massachusetts General Hospital, Boston 02114.
出版信息
Science. 1988 Sep 23;241(4873):1667-9. doi: 10.1126/science.241.4873.1667.
Abstract
A defective herpes simplex virus 1 (HSV-1) vector, pHSVlac, has been developed that contains a transcription unit that places the Escherichia coli lacZ gene under the control of the HSV-1 immediate early 4/5 promoter. The vector pHSVlac was propagated with the HSV-1 temperature-sensitive mutant ts K as helper virus. Infection of neurons from rat superior cervical ganglia and dorsal root ganglia in primary culture resulted in stable expression of high levels of beta-galactosidase without cell death. These HSV-1 vectors should be useful for introducing genes into postmitotic cells, such as neurons, in vitro and in vivo.
摘要
一种有缺陷的单纯疱疹病毒1型（HSV-1）载体pHSVlac已被研发出来，它包含一个转录单元，该转录单元使大肠杆菌lacZ基因置于HSV-1立即早期4/5启动子的控制之下。载体pHSVlac与HSV-1温度敏感突变体tsK作为辅助病毒一起增殖。对原代培养的大鼠颈上神经节和背根神经节中的神经元进行感染，导致高水平的β-半乳糖苷酶稳定表达且无细胞死亡。这些HSV-1载体在体外和体内将基因导入有丝分裂后细胞（如神经元）中应该是有用的。
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