MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150001, China.
Centre for Protolife Research and Centre for Organized Matter Chemistry, School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK.
Small. 2017 Jun;13(22). doi: 10.1002/smll.201700467. Epub 2017 Apr 25.
Controlled membrane fusion of proteinosome-based protocells is achieved via a hydrogel-mediated process involving dynamic covalent binding, self-healing, and membrane reconfiguration at the contact interface. The rate of proteinosome fusion is dependent on dynamic Schiff base covalent interchange, and is accelerated in the presence of encapsulated glucose oxidase and glucose, or inhibited with cinnamyl aldehyde due to enzyme-mediated decreases in pH or competitive covalent binding, respectively. The coordinated fusion of the proteinosomes leads to the concomitant transportation and redistribution of entrapped payloads such as DNA and dextran. Silica colloids with amino-functionalized surfaces undergo partial fusion with the proteinosomes via a similar dynamic hydrogel-mediated mechanism. Overall, the strategy provides opportunities for the development of interacting colloidal objects, control of collective behavior in soft matter microcompartmentalized systems, and increased complexity in synthetic protocell communities.
基于蛋白体的原细胞的受控膜融合是通过水凝胶介导的过程实现的，该过程涉及动态共价键合、自修复以及接触界面处的膜重构。蛋白体融合的速度取决于动态席夫碱共价交换，并且在存在包封的葡萄糖氧化酶和葡萄糖的情况下会加速，或者由于酶介导的 pH 值降低或竞争性共价键合，分别用肉桂醛抑制。蛋白体的协调融合导致包封的有效载荷（如 DNA 和葡聚糖）的伴随运输和再分配。表面具有氨基官能化的二氧化硅胶体通过类似的动态水凝胶介导的机制与蛋白体部分融合。总的来说，该策略为相互作用的胶体物体的发展、软物质微分隔系统中集体行为的控制以及合成原细胞群落的复杂性增加提供了机会。
