Mohamadi Nejad, Kazemi S Maryam, Mohammadian Mahshid, Toofani Milani Attabak, Moradi Younes, Yasemi Maryam, Ebrahimi far Meysam, Mazloumi Tabrizi Maral, Ebrahimi Shahmabadi Hasan, Akbarzadeh Khiyavi Azim
Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Email:
Asian Pac J Cancer Prev. 2017 Mar 1;18(3):629-632. doi: 10.22034/APJCP.2017.18.3.629.
Cancer is one of the most important issues in modern medicine and the most common cause of death after cardiovascular diseases in many countries. Brain cancer is one of the most common causes of cancer death among men and women, ranking third. Chemotherapeutic drugs that aim to prevent uncontrolled proliferation of cells in tissues of the body and induce apoptosis of tumor cells are prominent candidates for development. Since cisplatin has an apoptosisinducing role, it is widely used as an anticancer agent. In this research, toxicity of cisplatin was studied with the C6 rat glioma cell lined using the MTT method. In addition, nanoparticles underwent SEM microscopic imaging. Particle average size, size distribution, polydispersity index (PDI) and zeta potential of poly butyl cyanoacrylate nanoparticles were found to be 222 nm, 0.470 ± 0.04 and 5.1 ± 0.2 mV, respectively. The results showed that nanoconjugates of cisplatin have more cytotoxic effects on C6 cells than the free drug (P<0.05), pointing to an enhanced potential of the synthesized nano-particles as a new nanocarrier for chemotherapy.
癌症是现代医学中最重要的问题之一，在许多国家是仅次于心血管疾病的最常见死因。脑癌是男性和女性癌症死亡的最常见原因之一，排名第三。旨在防止体内组织细胞不受控制地增殖并诱导肿瘤细胞凋亡的化疗药物是开发的主要候选药物。由于顺铂具有诱导凋亡的作用，它被广泛用作抗癌药物。在本研究中，使用MTT法对C6大鼠胶质瘤细胞系研究了顺铂的毒性。此外，对纳米颗粒进行了扫描电子显微镜成像。聚氰基丙烯酸丁酯纳米颗粒的平均粒径、粒径分布、多分散指数（PDI）和zeta电位分别为222nm、0.470±0.04和5.1±0.2mV。结果表明，顺铂纳米缀合物对C6细胞的细胞毒性作用比游离药物更强（P<0.05），这表明合成的纳米颗粒作为一种新的化疗纳米载体具有增强的潜力。
