Garcia R C, Segal A W
Department of Medicine, Faculty of Clinical Sciences, University College London, U.K.
Biochem J. 1988 Jun 15;252(3):901-4. doi: 10.1042/bj2520901.
Cytochrome b-245, the only clearly identified component of the microbicidal oxidase system of phagocytes, is a heterodimer consisting of a 23 kDa (alpha) and a 76-92 kDa (beta) subunit. This study was conducted to examine whether, in common with a number of proteins, the subunits of the cytochrome were phosphorylated upon activation of the oxidase. Both subunits were phosphorylated after activation of neutrophils or macrophages with phorbol myristate acetate or a phagocytic stimulus, although the time course of this process did not parallel that of the oxidase. Phosphorylation of these proteins was normal in cells from two patients with autosomal recessive chronic granulomatous disease, in whom phosphorylation of a 47 kDa protein is defective.
细胞色素b - 245是吞噬细胞杀菌氧化酶系统中唯一明确鉴定出的成分，是一种异源二聚体，由一个23 kDa（α）亚基和一个76 - 92 kDa（β）亚基组成。本研究旨在检测细胞色素的亚基在氧化酶激活后是否与许多蛋白质一样发生磷酸化。在用佛波酯肉豆蔻酸酯乙酸盐或吞噬刺激激活中性粒细胞或巨噬细胞后，两个亚基均发生了磷酸化，尽管这一过程的时间进程与氧化酶的不同。在两名常染色体隐性慢性肉芽肿病患者的细胞中，这些蛋白质的磷酸化是正常的，而在这两名患者中，一种47 kDa蛋白质的磷酸化存在缺陷。
