反硝化副球菌呼吸链中抗霉素和杀稻瘟菌素的独立结合位点及其在其他反硝化细菌中的存在情况
Separate binding sites for antimycin and mucidin in the respiratory chain of the bacterium Paracoccus denitrificans and their occurrence in other denitrificans bacteria.
作者信息
Kucera I, Hedbávný R, Dadák V
机构信息
Department of Biochemistry, Faculty of Science, J. E. Purkynĕ University, Brno, Czechoslovakia.
出版信息
Biochem J. 1988 Jun 15;252(3):905-8. doi: 10.1042/bj2520905.
Abstract
By means of the method of fluorimetric titration it has been shown that mucidin does not affect the attachment of antimycin to membranes from anaerobically grown Paracoccus denitrificans. The fluorimetric titration with antimycin can be used in the determination of the amount of the cytochrome bc1 complex in the membrane. In cells inhibited with antimycin, the oxidation of cytochromes c was accompanied by the reduction of cytochrome b; in the presence of mucidin this effect did not take place. The results, which indicated a difference in binding sites, were interpreted in terms of the Q-cycle [Mitchell (1976) J. Theor. Biol. 62, 327-367; Trumpower (1981) Biochim. Biophys. Acta 639, 129-155]. Comparable sensitivity towards antimycin and mucidin was shown by other typical denitrifying bacteria: Pseudomonas stutzeri and Alcaligenes xylosoidans, subspecies denitrificans.
摘要
通过荧光滴定法已表明，粘菌素不影响抗霉素与厌氧生长的反硝化副球菌膜的结合。用抗霉素进行的荧光滴定可用于测定膜中细胞色素bc1复合物的含量。在用抗霉素抑制的细胞中，细胞色素c的氧化伴随着细胞色素b的还原；在粘菌素存在的情况下，这种效应未发生。这些表明结合位点存在差异的结果，根据Q循环[米切尔（1976年）《理论生物学杂志》62卷，327 - 367页；特朗普沃尔（1981年）《生物化学与生物物理学报》639卷，129 - 155页]进行了解释。其他典型的反硝化细菌：施氏假单胞菌和木糖氧化产碱菌反硝化亚种，对抗霉素和粘菌素表现出类似的敏感性。
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