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[Plasmids containing 2 new variants of the E.coli lacZ gene].

作者信息

Khudiakov Iu E, Kalinina T I, Nepliueva V S, Smirnov V D

出版信息

Bioorg Khim. 1988 Apr;14(4):478-83.

PMID:2844193
Abstract

New plasmids containing partially deleted lacZ genes were obtained. These genes determine high-level synthesis of polypeptides of molecular mass 43-45 and 49-51 kD under the control of the lambda phage PR-promoter; inspite of the deletion, E. coli cells carrying new plasmids were found to possess beta-galactosidase activity. Use of these plasmids as new expression vectors is suggested.

摘要

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