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一种在与限制性酶切位点末端相对应的DNA序列中诱导C----T点突变的方法

[A method for inducing the point C----T transitions in DNA sequences corresponding to the ends of restriction sites].

作者信息

Medvedev O A, Timchenko T V, Dianov G L

出版信息

Bioorg Khim. 1988 May;14(5):694-6.

PMID:2844195
Abstract

A new method for inducing of C----T substitutions into cytosine-containing restriction sites is developed. The method, based on the selective modification of cytosine residues in DNA sticky ends by sodium bisulfite, was illustrated by induction of a base substitution (C----T at the BamHI site of pBR322 plasmid DNA.

摘要

开发了一种将C→T取代引入含胞嘧啶的限制位点的新方法。该方法基于亚硫酸氢钠对DNA粘性末端胞嘧啶残基的选择性修饰，通过在pBR322质粒DNA的BamHI位点诱导碱基取代（C→T）进行了说明。

相似文献

1
[A method for inducing the point C----T transitions in DNA sequences corresponding to the ends of restriction sites].一种在与限制性酶切位点末端相对应的DNA序列中诱导C----T点突变的方法
Bioorg Khim. 1988 May;14(5):694-6.
2
[Localized mutagenesis of the tetracycline gene in the plasmid pBR322 induced by sodium bisulfite in vitro].[亚硫酸氢钠体外诱导质粒pBR322中四环素基因的定位诱变]
Mol Gen Mikrobiol Virusol. 1985 Aug(8):21-6.
3
Cluster of point mutations predetermined by a quasipalindromic nucleotide sequence in plasmid pBR322 DNA.质粒pBR322 DNA中由准回文核苷酸序列预先决定的点突变簇。
FEBS Lett. 1990 Feb 12;261(1):28-30. doi: 10.1016/0014-5793(90)80628-v.
4
[Mutations predetermined by the primary structure of DNA].[由DNA一级结构预先决定的突变]
Genetika. 1986 Oct;22(10):2398-407.
5
[Directed modification of the Tcr gene region of the plasmid pBR322 using complementary single-stranded DNA fragments carrying alkylating groups].[使用携带烷基化基团的互补单链DNA片段对质粒pBR322的Tcr基因区域进行定向修饰]
Mol Biol (Mosk). 1984 Jul-Aug;18(4):1081-9.
6
In vitro sodium bisulfite mutagenesis of restriction endonuclease recognition sites.限制性内切酶识别位点的体外亚硫酸氢钠诱变
Anal Biochem. 1987 May 15;163(1):79-87. doi: 10.1016/0003-2697(87)90095-9.
7
[Effective method of oligonucleotide-controlled mutagenesis of DNA fragments].[DNA片段的寡核苷酸控制诱变的有效方法]
Bioorg Khim. 1985 May;11(5):621-7.
8
[Site-specific deletions of vector plasmid pBR322].
Dokl Akad Nauk SSSR. 1980;253(5):1232-5.
9
[The plasmid carrying the temperature-sensitive mutation in the DNa-methylase gene of the PStI system: effect on host cells at nonpermissive temperature].[携带PStI系统DNA甲基化酶基因温度敏感突变的质粒：在非允许温度下对宿主细胞的影响]
Genetika. 1999 May;35(5):574-86.
10
[Induction of repetitive nucleotide sequences. The probable mechanisms of genome evolution and gene conversion].
Genetika. 1984 Aug;20(8):1244-54.

引用本文的文献

1
Repair of uracil residues closely spaced on the opposite strands of plasmid DNA results in double-strand break and deletion formation.修复质粒DNA相反链上紧密间隔的尿嘧啶残基会导致双链断裂和缺失形成。
Mol Gen Genet. 1991 Mar;225(3):448-52. doi: 10.1007/BF00261686.