Zhang Di, Zhang Kaiqi, Su Weiheng, Zhao Yuan, Ma Xin, Qian Gong, Qu Guijuan, Pei Zhihua, Liu Shuming, Ma Hongxia
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, PR China.
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, PR China.
Microb Pathog. 2017 Jun;107:430-435. doi: 10.1016/j.micpath.2017.04.031. Epub 2017 Apr 23.
Enterotoxigenic Escherichia coli (ETEC)-induced diarrhea is a complex pathological process, involving ion channel regulation and water efflux. While the mechanism underlying water efflux in ETEC-induced diarrhea is still largely unknown, aquaporins (AQPs) play important roles in transcellular water movement, but their expression profile has not been demonstrated in the murine small intestine. We identified AQP3 expression in the jejunum, but not the duodenum or ileum, using reverse transcription PCR and western blotting. Immunohistochemistry showed that AQP3 localized to the jejunum villi epithelial cells. Using an ETEC-induced murine diarrhea model, we demonstrated that both AQP3 mRNA expression and protein concentration in the jejunum were gradually but significantly decreased over 7 d compared with controls. These results suggested impaired water influx also plays an important role in ETEC-induced diarrhea.
产肠毒素大肠杆菌（ETEC）引起的腹泻是一个复杂的病理过程，涉及离子通道调节和水外流。虽然ETEC引起腹泻时水外流的潜在机制仍大多未知，但水通道蛋白（AQPs）在跨细胞水转运中起重要作用，但其在小鼠小肠中的表达谱尚未得到证实。我们使用逆转录PCR和蛋白质印迹法鉴定出空肠中有AQP3表达，而十二指肠和回肠中没有。免疫组织化学显示AQP3定位于空肠绒毛上皮细胞。使用ETEC诱导的小鼠腹泻模型，我们证明与对照组相比，空肠中AQP3 mRNA表达和蛋白质浓度在7天内逐渐但显著降低。这些结果表明水流入受损在ETEC引起的腹泻中也起重要作用。
