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不同环境条件下[具体生物名称未给出]转录因子的基因表达谱分析

Gene Expression Profiling of Transcription Factors of under Different Environmental Conditions.

作者信息

De la Cruz Miguel A, Ares Miguel A, von Bargen Kristine, Panunzi Leonardo G, Martínez-Cruz Jessica, Valdez-Salazar Hilda A, Jiménez-Galicia César, Torres Javier

机构信息

Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatria, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro SocialMexico City, Mexico.

Phico Therapeutics LtdCambridge, UK.

出版信息

Front Microbiol. 2017 Apr 10;8:615. doi: 10.3389/fmicb.2017.00615. eCollection 2017.

DOI:10.3389/fmicb.2017.00615
PMID:28443084
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5385360/
Abstract

is a Gram-negative bacterium that colonizes the human gastric mucosa and causes peptic ulcers and gastric carcinoma. strain 26695 has a small genome (1.67 Mb), which codes for few known transcriptional regulators that control bacterial metabolism and virulence. We analyzed by qRT-PCR the expression of 16 transcriptional regulators in 26695, including the three sigma factors under different environmental conditions. When bacteria were exposed to acidic pH, urea, nickel, or iron, the sigma factors were differentially expressed with a particularly strong induction of . The regulatory genes , and were highly induced in the presence of urea, nickel, and iron. In terms of biofilm formation , and were induced in sessile bacteria. Transcriptional expression levels of , and were increased in contact with AGS epithelial cells. Kanamycin, chloramphenicol, and tetracycline increased or decreased expression of regulatory genes, showing that these antibiotics affect the transcription of . Our data indicate that environmental cues which may be present in the human stomach modulate transcription.

摘要

是一种革兰氏阴性菌，它定殖于人类胃黏膜并导致消化性溃疡和胃癌。26695菌株有一个小基因组（1.67 Mb），该基因组编码很少已知的控制细菌代谢和毒力的转录调节因子。我们通过qRT-PCR分析了26695中16种转录调节因子的表达，包括在不同环境条件下的三种sigma因子。当细菌暴露于酸性pH、尿素、镍或铁时，sigma因子差异表达，其中 有特别强烈的诱导。调节基因 、 和 在有尿素、镍和铁存在时被高度诱导。就生物膜形成而言， 和 在固着细菌中被诱导。 、 和 与AGS上皮细胞接触时转录表达水平增加。卡那霉素、氯霉素和四环素增加或降低调节基因的表达，表明这些抗生素影响 的转录。我们的数据表明，人类胃中可能存在的环境线索调节 转录。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabe/5385360/2b7e401195db/fmicb-08-00615-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabe/5385360/7983c34c32d8/fmicb-08-00615-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabe/5385360/65d9e92f3808/fmicb-08-00615-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabe/5385360/c36c1dd3f6a8/fmicb-08-00615-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabe/5385360/8371008c0794/fmicb-08-00615-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabe/5385360/2b7e401195db/fmicb-08-00615-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabe/5385360/7983c34c32d8/fmicb-08-00615-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabe/5385360/65d9e92f3808/fmicb-08-00615-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabe/5385360/c36c1dd3f6a8/fmicb-08-00615-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabe/5385360/8371008c0794/fmicb-08-00615-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabe/5385360/2b7e401195db/fmicb-08-00615-g005.jpg

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