Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
Int Microbiol. 2023 Nov;26(4):907-915. doi: 10.1007/s10123-023-00347-z. Epub 2023 Mar 21.
The prevention of biofilm formation plays a pivotal role in managing Helicobacter pylori inside the body and the environment. This study showed in vitro potentials of two recently isolated probiotic strains, Bacillus sp. 1630F and Enterococcus sp. 7C37, to form biofilm and combat H. pylori attachment to the abiotic and biotic surfaces. Lactobacillus casei and Bifidobacterium bifidum were used as the reference probiotics. The biofilm rates were the highest in the solid-liquid interface for Lactobacillus and Bifidobacterium and the air-liquid interface for Bacillus and Enterococcus. The highest tolerances to the environmental conditions were observed during the biofilm formations of Enterococcus and Bifidobacterium (pH), Enterococcus and Bacillus (bile), and Bifidobacterium and Lactobacillus (NaCl) on the polystyrene and glass substratum, respectively. Biofilms occurred more quickly by Bacillus and Enterococcus strains than reference strains on the polystyrene and glass substratum, respectively. Enterococcus (competition) and Bacillus (exclusion) achieved the most inhibition of H. pylori biofilm formations on the polystyrene and AGS cells, respectively. Expression of luxS was promoted by Bacillus (exclusion, 3.2 fold) and Enterococcus (competition, 2.0 fold). Expression of ropD was decreased when H. pylori biofilm was excluded by Bacillus (0.4 fold) and Enterococcus (0.2 fold) cells. This study demonstrated the ability of Bacillus and Enterococcus probiotic bacteria to form biofilm and combat H. pylori biofilm formation.
生物膜形成的预防在管理体内和环境中的幽门螺杆菌方面起着关键作用。本研究显示了两种最近分离的益生菌菌株，芽孢杆菌 1630F 和肠球菌 7C37，在体外形成生物膜和抵抗幽门螺杆菌附着于非生物和生物表面的潜力。干酪乳杆菌和双歧杆菌被用作参考益生菌。在固体-液体界面，乳酸菌和双歧杆菌的生物膜率最高，在气-液界面，芽孢杆菌和肠球菌的生物膜率最高。在聚苯乙烯和玻璃基质上，观察到肠球菌和双歧杆菌（pH）、肠球菌和芽孢杆菌（胆汁）以及双歧杆菌和乳酸菌（NaCl）的生物膜形成过程中，肠球菌和双歧杆菌对环境条件的耐受性最高。与参考菌株相比，芽孢杆菌和肠球菌菌株在聚苯乙烯和玻璃基质上更快地形成生物膜。肠球菌（竞争）和芽孢杆菌（排除）分别在聚苯乙烯和AGS 细胞上对幽门螺杆菌生物膜形成的抑制作用最大。luxS 的表达被芽孢杆菌（排斥，3.2 倍）和肠球菌（竞争，2.0 倍）促进。当幽门螺杆菌生物膜被芽孢杆菌（0.4 倍）和肠球菌（0.2 倍）细胞排除时，ropD 的表达降低。本研究表明芽孢杆菌和肠球菌益生菌能够形成生物膜并抵抗幽门螺杆菌生物膜的形成。
