Acar Yahya Ayhan, Karakuş Yılmaz Banu, Çelik Duygu Sultan, Çevik Erdem, Topçu Hatice, Özsoy Şule, Haklıgör Aylin, Çınar Orhan
Clinic of Emergency Medicine, Etimesgut Military Hospital, Ankara, Turkey.
Clinic of Emergency Medicine, Hamidiye Şişli Etfal Training and Research Hospital, İstanbul, Turkey.
Balkan Med J. 2017 May 5;34(3):212-218. doi: 10.4274/balkanmedj.2016.0782. Epub 2017 Apr 6.
Therapeutic hypothermia was showed to improve neurologic outcome but current therapeutic hypothermia techniques have limitations. Novel techniques such as transpulmonary hypothermia with cooled oxygen inhalation may be beneficial.
To evaluate the performance of transthoracic hypothermia with cooled medical oxygen inhalation as a therapeutic hypothermia method.
Animal experimentation.
A total of 36 adult male Wistar-Hannover rats were used in this research. Rats were randomised into four groups: group 1, Cooled oxygen group; group 2, IV cold fluid group; group 3, Surface cooling group; group 4, control group. No hypothermia method was applied in the control group. Hypothermia techniques were administered in the other three groups until the targeted core temperature was maintained. The target temperature was continued for one hour at 32-34 °C. After that, rats were heated up with hot blankets. Once the rectal temperature reached 38 °C, rats were euthanised. The main outcomes were the rate of temperature decrease (°C per minute) (S) and the time required to reach the target body temperature (T).
All rats survived the study protocol. When compared to the control group, T and S values were better in the cooled medical oxygen inhalation group (p<0.001). The IV cold fluid group had lower S values and higher T values compared to the cooled oxygen group (p<0.001, and p=0.003, respectively). There was no meaningful pathology in the histological samples in any group.
As an easy-to-use and inexpensive method, cooled oxygen inhalation may be a beneficial hypothermia technique.
治疗性低温已被证明可改善神经功能结局，但目前的治疗性低温技术存在局限性。诸如吸入冷却氧气的经肺低温等新技术可能有益。
评估吸入冷却医用氧气的经胸低温作为一种治疗性低温方法的性能。
动物实验。
本研究共使用36只成年雄性Wistar-Hannover大鼠。大鼠被随机分为四组：第1组，冷却氧气组；第2组，静脉输注冷液体组；第3组，体表冷却组；第4组，对照组。对照组不应用低温方法。在其他三组中实施低温技术，直至维持目标核心体温。目标温度在32 - 34°C持续1小时。之后，用热毯使大鼠升温。一旦直肠温度达到38°C，将大鼠安乐死。主要结局为降温速率（每分钟°C）（S）和达到目标体温所需时间（T）。
所有大鼠均存活至研究方案结束。与对照组相比，吸入冷却医用氧气组的T和S值更好（p<0.001）。与冷却氧气组相比，静脉输注冷液体组的S值更低，T值更高（分别为p<0.001和p = 0.003）。任何组的组织学样本中均无有意义的病理学改变。
作为一种易于使用且成本低廉的方法，吸入冷却氧气可能是一种有益的低温技术。
