Mohanta Tapan Kumar, Bae Hanhong
Free Major of Natural Sciences, School of Basic Studies, Yeungnam University, Gyeongsan, Gyeongsangbuk-do, 38541, Republic of Korea.
School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongsangbuk-do, 38541, Republic of Korea.
3 Biotech. 2017 May;7(1):51. doi: 10.1007/s13205-017-0689-6. Epub 2017 Apr 25.
Auxin signaling events in plants play important role in developmental regulation as well as gravitropic responses and plays crucial role in the development of root, lateral root and root hairs. The gene that is known to be most important in the development of root, lateral root and root hairs is commonly known as auxin efflux carrier (PIN). Being commonly known as orphan plant, the genome sequence of Eleusine coracana is not known yet, and hence it was very difficult to conduct advanced research in root development in this plant. As PIN gene plays crucial role in root development, to have some advanced study we proposed to clone the PIN genes from E. coracana. We cloned two PIN genes in E. coracana and named them as EcPIN1a and EcPIN1b. The coding sequence (CDS) of EcPIN1a was 1779 bp and EcPIN1b was 1788 bp long that encodes for 593 and 596 amino acids, respectively. In-silico analysis shows the presence of transmembrane domain in EcPIN1a and EcPIN1b protein. Multiple sequence alignment of EcPIN1a and EcPIN1b protein shows the presence of several conserved motifs. Phylogenetic analysis of EcPIN1a and EcPIN1b grouped with the PIN gene of monocot plant Oryza sativa. This shows that EcPIN genes were monocot specific, and closely match with the PIN genes of O. sativa. The transcript analysis of EcPIN1a gene in leaf tissue shows gradual up-regulation from 7th to 28th days of developmental time period while the transcript level was found to be lower in root tissue. The transcript abundance of EcPIN1b was not detected. Gradual up-regulation of EcPIN1a gene in developmental stages signifies its important role in root development in E. coracana.
植物中的生长素信号传导事件在发育调控以及向重力性反应中发挥重要作用，并且在根、侧根和根毛的发育中起关键作用。已知在根、侧根和根毛发育中最重要的基因通常被称为生长素外流载体（PIN）。由于薏苡通常被称为孤生植物，其基因组序列尚不清楚，因此很难对该植物的根发育进行深入研究。由于PIN基因在根发育中起关键作用，为了进行一些深入研究，我们提议从薏苡中克隆PIN基因。我们在薏苡中克隆了两个PIN基因，并将它们命名为EcPIN1a和EcPIN1b。EcPIN1a的编码序列（CDS）为1779 bp，EcPIN1b为1788 bp长，分别编码593和596个氨基酸。电子分析表明EcPIN1a和EcPIN1b蛋白中存在跨膜结构域。EcPIN1a和EcPIN1b蛋白的多序列比对显示存在几个保守基序。EcPIN1a和EcPIN1b的系统发育分析与单子叶植物水稻的PIN基因聚类。这表明EcPIN基因是单子叶植物特有的，并且与水稻的PIN基因密切匹配。叶组织中EcPIN1a基因的转录分析显示在发育时间段的第7天到第28天逐渐上调，而在根组织中发现转录水平较低。未检测到EcPIN1b的转录丰度。EcPIN1a基因在发育阶段的逐渐上调表明其在薏苡根发育中的重要作用。
