Piao Songzhe, Wang Yue, Lee Young Ju, Hong Seungsoo, Jeong Yoonchan, Oh Seung-June
Department of Urology, Seoul National University Hospital, Seoul, Korea.
Department of Urology, Post-Doctoral Research Center, Yanbian University Hospital, Yanji, China.
Int Neurourol J. 2017 Apr;21(Suppl 1):S17-23. doi: 10.5213/inj.1734858.429. Epub 2017 Apr 21.
The aim of this study was to perform a histological analysis of the effect of a ytterbium-doped fiber (YDF) laser on oral buccal mucosa tissue to simulate its effect on the mucosa of the lower urinary tract.
A total of 90 8-week-old Sprague-Dawley rats were anesthetized with urethrane (1.2 g/kg intraperitoneally). A prespecified inner buccal mucosal site was irradiated with a YDF master-oscillator power amplifier (MOPA) system for 60 seconds, with output power settings of 0.5, 1, and 2 W, respectively, in 3 treatment groups. Specimens of irradiated tissue were harvested at 2 hours, 24 hours, 2 weeks, and 4 weeks after irradiation. The tissue specimens were stained with hematoxylin and eosin for histological analysis.
In the group treated with 0.5 W, basal cell elongation and vacuolization were observed at 2 hours and 24 hours after treatment, respectively. No evident injury was observed after 2 or 4 weeks. The group treated with 1 W presented partial basal layer separation, and even complete epidermal ablation, within 2 hours. At 24 hours after laser treatment, new capillaries on an edematous background of fibroblasts and myofibroblasts, as well as profuse infiltration of the neutrophils to the basal layer, were observed. Collagen deposition and reepithelization were observed in specimens taken 2 weeks and 4 weeks after treatment. The group treated with 2 W presented bigger and deeper injuries at 2 hours after irradiation. Meanwhile, subepidermal bullae with full-thickness epidermal necrosis and underlying inflammatory infiltrate were observed 24 hours after treatment. The presence of fibrous connective tissue and collagen deposition were observed 2 weeks and 4 weeks after the treatment.
To our knowledge, this is the first report regarding the effect of a YDF laser on living tissue. Our study demonstrated that the typical histological findings of the tissue reaction to the YDF MOPA apparatus were very similar to those associated with thermal injuries. The extent and degree of tissue damage increased proportionally to the output power.
本研究旨在对掺镱光纤（YDF）激光作用于口腔颊黏膜组织的效果进行组织学分析，以模拟其对下泌尿道黏膜的影响。
总共90只8周龄的Sprague-Dawley大鼠用氨基甲酸乙酯（1.2 g/kg腹腔注射）麻醉。在3个治疗组中，分别用YDF主振荡器功率放大器（MOPA）系统以0.5、1和2 W的输出功率设置对预定的口腔颊黏膜部位照射60秒。在照射后2小时、24小时、2周和4周采集照射组织的标本。组织标本用苏木精和伊红染色以进行组织学分析。
在0.5 W治疗组中，分别在治疗后2小时和24小时观察到基底细胞伸长和空泡化。2周或4周后未观察到明显损伤。1 W治疗组在2小时内出现部分基底层分离，甚至完全表皮剥脱。激光治疗后24小时，观察到在成纤维细胞和平滑肌成纤维细胞的水肿背景上有新的毛细血管，以及大量中性粒细胞浸润到基底层。在治疗后2周和4周采集的标本中观察到胶原沉积和上皮再形成。2 W治疗组在照射后2小时出现更大更深的损伤。同时，治疗后24小时观察到全层表皮坏死和下方炎症浸润的表皮下大疱。治疗后2周和4周观察到纤维结缔组织和胶原沉积的存在。
据我们所知，这是关于YDF激光对活体组织影响的首次报告。我们的研究表明，组织对YDF MOPA设备反应的典型组织学表现与热损伤相关的表现非常相似。组织损伤的程度和范围与输出功率成正比增加。
