脑钠肽与培养的大鼠血管平滑肌细胞中的心房钠尿肽受体相互作用。

Brain natriuretic peptide interacts with atrial natriuretic peptide receptor in cultured rat vascular smooth muscle cells.

作者信息

Hirata Y, Shichiri M, Emori T, Marumo F, Kangawa K, Matsuo H

机构信息

Hypertension-Endocrine Division, National Cardiovascular Center Research Institute, Osaka, Japan.

出版信息

FEBS Lett. 1988 Oct 10;238(2):415-8. doi: 10.1016/0014-5793(88)80523-4.

DOI:10.1016/0014-5793(88)80523-4

PMID:2844607

Abstract

The effect of synthetic porcine brain natriuretic peptide (pBNP), a novel brain peptide with sequence homology to alpha-human atrial natriuretic peptide (hANP), on receptor binding and cGMP generation, was studied in cultured rat vascular smooth muscle cells (VSMC) and compared with that of alpha-hANP. 125I-pBNP bound to the cells in a time-dependent manner similar to that of 125I-alpha-hANP. Scatchard analysis indicated a single class of binding sites for pBNP with affinity and capacity identical to those of alpha-hANP. pBNP and alpha-hANP were almost equipotent in inhibiting the binding of either radioligand and stimulating intracellular cGMP generation. These data indicate that BNP and ANP interact with the same receptor sites to activate guanylate cyclase in rat VSMC.

摘要

研究了合成猪脑钠肽（pBNP），一种与α-人心房钠尿肽（hANP）具有序列同源性的新型脑肽，对培养的大鼠血管平滑肌细胞（VSMC）中受体结合和环鸟苷酸（cGMP）生成的影响，并与α-hANP进行了比较。125I-pBNP以与125I-α-hANP相似的时间依赖性方式与细胞结合。Scatchard分析表明pBNP有一类结合位点，其亲和力和容量与α-hANP相同。pBNP和α-hANP在抑制任何一种放射性配体的结合以及刺激细胞内cGMP生成方面几乎具有同等效力。这些数据表明，脑钠肽（BNP）和心房钠尿肽（ANP）与相同的受体位点相互作用，以激活大鼠VSMC中的鸟苷酸环化酶。