12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯干扰布氏毛霉孢子囊孢子的萌发。
12-O-tetradecanoyl phorbol-13-acetate interferes with germination of Phycomyces blakesleeanus sporangiospores.
作者信息
Carrillo-Rayas T, Garcia-Soto J, Martinez-Cadena G
机构信息
Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Mexico.
出版信息
FEBS Lett. 1988 Oct 10;238(2):441-4. doi: 10.1016/0014-5793(88)80528-3.
The presence of protein kinase C (PKC), a key enzyme in signal transduction, has not been investigated in fungal cells. The phorbol ester TPA, an activator of PKC, may be used as an indicator of the presence and role of PKC in Phycomyces blakesleeanus spores. Activation of spore germination by acetate was prevented by 6 nM TPA. The TPA analog 4 alpha PDD, an ineffective activator of PKC, did not affect spore germination. 3 mM dbcAMP, on the other hand, reversed the inhibition of germination caused by TPA. TPA-stimulated protein kinase activity was detected in spores. The possible relationship between PKC and the increased levels of cAMP that accompany the induction of spore germination is discussed.
蛋白激酶C（PKC）作为信号转导中的关键酶，其在真菌细胞中的存在情况尚未得到研究。佛波酯TPA是PKC的激活剂，可作为PKC在布氏毛霉孢子中的存在及作用的指示剂。6 nM TPA可阻止醋酸盐对孢子萌发的激活作用。TPA类似物4αPDD是一种无效的PKC激活剂，不影响孢子萌发。另一方面，3 mM dbcAMP可逆转TPA对萌发的抑制作用。在孢子中检测到TPA刺激的蛋白激酶活性。文中讨论了PKC与孢子萌发诱导过程中伴随的cAMP水平升高之间的可能关系。
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