Mante Priscilla Kolibea, Adongo Donatus Wewura, Woode Eric
Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
University of Health and Allied Sciences, Ho, Ghana.
BMC Res Notes. 2017 Apr 26;10(1):167. doi: 10.1186/s13104-017-2488-x.
Antiaris toxicaria has previously shown anticonvulsant activity in acute animal models of epilepsy. The aqueous extract (AAE) was further investigated for activity in kindling with pentylenetetrazole and administration of pilocarpine and kainic acid which mimic temporal lobe epilepsy in various animal species.
ICR mice and Sprague-Dawley rats were pre-treated with AAE (200-800 mg kg) and convulsive episodes induced using pentylenetetrazole, pilocarpine and kainic acid. The potential of AAE to prevent or delay onset and alter duration of seizures were measured. In addition, damage to hippocampal cells was assessed in kainic acid-induced status epilepticus test. 800 mg kg of the extract suppressed the kindled seizure significantly (P < 0.05) as did diazepam. AAE also produced significant effect (P < 0.01) on latency to first myoclonic jerks and on total duration of seizures. The latency to onset of wet dog shakes was increased significantly (P < 0.05) by AAE on kainic acid administration. Carbamazepine and Nifedipine (30 mg kg) also delayed the onset. Histopathological examination of brain sections showed no protective effect on hippocampal cells by AAE and nifedipine. Carbamazepine offered better preservation of hippocampal cells in the CA1, CA2 and CA3 regions.
Antiaris toxicaria may be effective in controlling temporal lobe seizures in rodents.
之前已证明见血封喉在癫痫急性动物模型中具有抗惊厥活性。进一步研究了其水提取物（AAE）在戊四氮点燃以及给予匹鲁卡品和 kainic 酸（可模拟多种动物物种的颞叶癫痫）方面的活性。
用 AAE（200 - 800mg/kg）预处理 ICR 小鼠和 Sprague - Dawley 大鼠，然后使用戊四氮、匹鲁卡品和 kainic 酸诱导惊厥发作。测量了 AAE 预防或延迟发作起始以及改变癫痫持续时间的潜力。此外，在 kainic 酸诱导的癫痫持续状态试验中评估了对海马细胞的损伤。800mg/kg 的提取物与地西泮一样，显著抑制了点燃性癫痫发作（P < 0.05）。AAE 对首次肌阵挛抽搐的潜伏期和癫痫发作的总持续时间也产生了显著影响（P < 0.01）。给予 kainic 酸时，AAE 显著增加了湿狗样抖动发作的潜伏期（P < 0.05）。卡马西平和硝苯地平（30mg/kg）也延迟了发作起始。脑切片的组织病理学检查显示，AAE 和硝苯地平对海马细胞没有保护作用。卡马西平在 CA1、CA2 和 CA3 区域对海马细胞的保存效果更好。
见血封喉可能对控制啮齿动物的颞叶癫痫有效。
