Lu Xue-Ying, Ye Li-Lin, Shi Yu-Ye, Ding Yi-Han, Li Yu-Feng
Department of Hematology, Huaian First People's Hospital,Huaian 223300, Jiangsu Province, China.
Department of Oncology, Jiangsu Shengze Hospital,Wujiang 215228,Jiangsu Province,China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017 Apr;25(2):412-417. doi: 10.7534/j.issn.1009-2137.2017.02.019.
To study the effect of homoharringtonine (HHT) alone or combined with 3-methyladenine (3-MA) , an autophagy inhibitor, on the apoptosis and autophagy of K562 cells.
K562 cells were treated with HHT(10 ng/ml) or HHT(10 ng/ml) combined with 3-MA (1.5 mmol/L) for 1 to 8 days. The apoptosis of treated cells was tested by means of flow cytometry(FCM), and the autophagy levels were tested with RT-PCR, Western blot and electron microscopy.
In the early stage of HHT-treated group, the apoptosis rate increased and decreased later. Beclin1 mRNA expression level and the LC3II/I ratio were declined firstly and increased later in HHT group. While combining with autophagy inhibitor 3-MA, both the Beclin1 mRNA expression level and the LC3II/I ratio were declined continually during the treated period. The activated caspase-3 protein expression level was also raised sustainability during both HHT and 3-MA cultured period.
HHT can induce apoptosis of K562 cells, but the sustaining effect of HHT can induc autophagy of K562 cells, the combination of HHT with 3-MA may enhance the cytotoxicitic effect of HHT on K562 cells.
