再生障碍性贫血患者端粒长度、MRE11和Ku80的表达及其与发病机制的相关性

[Telomere Length, Expression of MRE11 and Ku80 in Patients with Aplastic Anemia and Their Correlation with Pathogenesis].

作者信息

Wang Yan, Xu Rui-Rong, DU Ying-Jun, Wang Jing-Yi, Liu Kui, Zheng Wei

机构信息

Department of Hematology, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China.

Department of Hematology, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China. E-mail: xrr18@ sina. com.

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017 Apr;25(2):503-509. doi: 10.7534/j.issn.1009-2137.2017.02.036.

DOI:10.7534/j.issn.1009-2137.2017.02.036

PMID:28446302

Abstract

OBJECTIVE

To detect the expression levels of MRE11 and Ku80 mRNA, and telomere length in bone marrow mononuclear cells of aplastic anemia(AA) patients, and to explore their correlation with pathogenesis of aplastic anemia.

METHODS

Bone marrow mononuclear cells were collected from 40 cases of AA and 20 normal controls for detecting mRNA expression of MRE11 and Ku80 and telomere length by using real-time quantitative polymerase chain reaction (qPCR), then MRE11, Ku80 and telomere length were analyzed for their correlation.

RESULTS

As compared with controls, the expression levels of MRE11 and Ku80 in patients with AA were significantly reduced, and the telomere length in patients with AA was obviously shortened, respectively (P<0. 05). The telomere length was significantly shorter in the persons aged ≥45 years in comparison with the AA patients and normal control younger than 45 years old (P<0.05). For the AA patients older than or equal to 45 years and less than 45 years in comparison with the controls at the same age, the telomere length was significantly shorter(P<0.05). The expression levels of MRE11 and Ku80 didn't correlate with telomere length (P>0.05). The mRNA expression level of MRE11 correlated positively and significantly with that of Ku80 (r=0.863, P<0.05).

CONCLUSION

The change of telomere length may play an important role in the pathogenesis and progression of aplastic anemia. The lower expression of MRE11 and Ku80 may be involved in the pathogenesis of aplastic anemia.

摘要

目的

检测再生障碍性贫血（AA）患者骨髓单个核细胞中MRE11和Ku80 mRNA表达水平及端粒长度，并探讨其与再生障碍性贫血发病机制的相关性。

方法

收集40例AA患者及20例正常对照者的骨髓单个核细胞，采用实时定量聚合酶链反应（qPCR）检测MRE11和Ku80 mRNA表达及端粒长度，分析MRE11、Ku80与端粒长度的相关性。

结果

与对照组相比，AA患者MRE11和Ku80表达水平显著降低，端粒长度明显缩短（P<0.05）。≥45岁者端粒长度明显短于<45岁的AA患者及正常对照者（P<0.05）。≥45岁及<45岁的AA患者与同年龄对照组相比，端粒长度均显著缩短（P<0.05）。MRE11和Ku80表达水平与端粒长度无相关性（P>0.05）。MRE11 mRNA表达水平与Ku80 mRNA表达水平呈显著正相关（r=0.863，P<0.05）。