Haddada H, Le Goffic N, Chandrasekaran K, de Vaux Saint Cyr C
Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Immunol Lett. 1988 Jul;18(3):225-9. doi: 10.1016/0165-2478(88)90023-5.
Simian virus 40 (SV40) large T antigen and p53 cellular protein were isolated from an SV40-transformed hamster cell line by immunoprecipitation with anti-T sera and purified by sodium dodecyl sulfate-gel electrophoresis. These two protein were tested in hamsters for the presence of SV40 transplantation rejection antigenic sites by in vivo transplantation rejection assay. The large T antigen immunized the hamsters against a challenge of SV40 tumor cells and the protected animals generated cytotoxic spleen cells. Hamsters immunized with the p53 cellular protein were not protected against SV40-induced tumor but there was some delay in the appearance of tumor.
通过用抗T血清进行免疫沉淀，从猿猴病毒40（SV40）转化的仓鼠细胞系中分离出SV40大T抗原和p53细胞蛋白，并通过十二烷基硫酸钠-凝胶电泳进行纯化。通过体内移植排斥试验在仓鼠中检测这两种蛋白是否存在SV40移植排斥抗原位点。大T抗原使仓鼠对SV40肿瘤细胞的攻击产生免疫，受保护的动物产生细胞毒性脾细胞。用p53细胞蛋白免疫的仓鼠未受到SV40诱导肿瘤的保护，但肿瘤出现有一定延迟。
