Volpe E A
Neoplasma. 1976;23(4):345-53.
The blocking of specific SV40-induced resistance of hamsters by the sera collected from 12 individual hamsters infected with SV40 when newborn was studied at different periods of primary virus-induced carcinogenesis. The serum samples were collected at the following periods of primary carcinogenesis: during the latent period (60 days after virus inoculation), at the day of primary tumor appearance, and 19-36 and 45-57 days after primary tumor appearance. For detection of the blocking activity of the collected sera the cells of transplantable SV40 test-tumor were pretreated in vitro with these sera and with control normal hamster sera, and then used for challenge in vivo in SV40-immunized and normal hamsters. With the use of such method, as a rule, no blocking activity of the serum samples collected at any time after the tumor appearance was observed. However, the sera obtained from 7 out of 12 of these hamsters during the latent period significantly decreased the resistance index of animals challenged in transplantation test with the serum pretreated tumor cells. Possible explanations of these findings are discussed.
在原发性病毒诱导的致癌作用的不同阶段，研究了从12只新生时感染SV40的仓鼠个体收集的血清对特定SV40诱导的仓鼠抗性的阻断作用。在原发性致癌作用的以下阶段收集血清样本：潜伏期（病毒接种后60天）、原发性肿瘤出现当天、原发性肿瘤出现后19 - 36天和45 - 57天。为了检测所收集血清的阻断活性，将可移植的SV40测试肿瘤细胞在体外先用这些血清和对照正常仓鼠血清进行预处理，然后用于在SV40免疫的仓鼠和正常仓鼠体内进行攻击试验。使用这种方法，通常在肿瘤出现后的任何时间收集的血清样本均未观察到阻断活性。然而，在潜伏期从这12只仓鼠中的7只获得的血清，在用血清预处理的肿瘤细胞进行移植试验中，显著降低了接受攻击的动物的抗性指数。讨论了这些发现的可能解释。
