Jeßberger Nadja, Rademacher Corinna, Krey Viktoria M, Dietrich Richard, Mohr Ann-Katrin, Böhm Maria-Elisabeth, Scherer Siegfried, Ehling-Schulz Monika, Märtlbauer Erwin
Department of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität MünchenOberschleißheim, Germany.
Functional Microbiology, Department of Pathobiology, Institute of Microbiology, University of Veterinary Medicine ViennaVienna, Austria.
Front Microbiol. 2017 Apr 12;8:627. doi: 10.3389/fmicb.2017.00627. eCollection 2017.
is a ubiquitous bacterial pathogen increasingly reported to be the causative agent of foodborne infections and intoxications. Since the enterotoxins linked to the diarrheal form of food poising are foremost produced in the human intestine, the toxic potential of enteropathogenic strains is difficult to predict from studies carried out under routine cultivation procedures. In this study, toxigenic properties of a panel of strains ( = 19) of diverse origin were compared using cell culture medium pre-incubated with CaCo-2 cells to mimic intestinal growth conditions. Shortly after contact of the bacteria with the simulated host environment, enterotoxin gene expression was activated and total protein secretion of all strains was accelerated. Although the signal stimulating enterotoxin production still needs to be elucidated, it could be shown that it originated from the CaCo-2 cells. Overall, our study demonstrates that the currently used methods in diagnostics, based on standard culture medium, are not allowing a conclusive prediction of the potential health risk related to a certain strain. Thus, these methods should be complemented by cultivation procedures that are simulating intestinal host conditions.
是一种普遍存在的细菌病原体，越来越多地被报道为食源性感染和中毒的病原体。由于与腹泻型食物中毒相关的肠毒素主要在人体肠道中产生，因此从常规培养程序下进行的研究很难预测肠道致病菌株的潜在毒性。在本研究中，使用预先与CaCo-2细胞一起孵育的细胞培养基来模拟肠道生长条件，比较了一组不同来源的菌株（n = 19）的产毒特性。细菌与模拟宿主环境接触后不久，肠毒素基因表达被激活，所有菌株的总蛋白分泌加速。尽管刺激肠毒素产生的信号仍有待阐明，但可以证明它源自CaCo-2细胞。总体而言，我们的研究表明，目前基于标准培养基的诊断方法无法对与某一菌株相关的潜在健康风险做出确定性预测。因此，这些方法应辅以模拟肠道宿主条件的培养程序。
