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感染印度小鲤(汉密尔顿,1822年)鱼鳍的1962年卡德里粘孢子虫(刺胞动物门,粘孢子虫纲,双壳目)的分子和系统发育特征

Molecular and phylogenetic characterization of Qadri, 1962 (Cnidaria, Myxosporea, Bivalvulida) infecting the fin of Indian minor carp (Hamilton, 1822).

作者信息

Kaur Harpreet, Gupta Aditya

机构信息

Department of Zoology, Panjab University, Chandigarh-160014, Chandigarh, India.

Department of Zoology and Environmental Sciences, Punjabi University, Patiala-147002, Punjab, India.

出版信息

Mol Biol Res Commun. 2017 Mar;6(1):13-21.

Abstract

A myxozoan parasite belonging to the genus Kudo, 1933 (Cnidaria, Myxosporea, Bivalvulida) was isolated from the fins of inhabiting Ranjit Sagar Wetland, Punjab, India. The plasmodium was 0.5-0.7 mm in diameter each containing 80- 100 number of myxospores. Myxospores were egg shaped to ovoidal in valvular view having bluntly pointed anterior and broad rounded posterior end measuring 8.36x 4.77 µm. Polar capsule was single, broadly pyriform in shape, measuring 4.77x3.98 µm in size containing a polar filament coiled perpendicular to the longitudinal axis of myxospore body making 7-9 turns. Blast analysis of 18S rDNA sequence of the isolate demonstrated 98% homogeneity with sp. (KU155561, Unpublished), followed by 91% with (KM252684) and sp. FCO (KR819273). The intensity of infection was recorded to be light as indicated by fin plasmodial index (FPI = 1). In the present study, has been described using 18S rRNA gene and phylogenetic analysis using MEGA.6.

摘要

一种属于 Kud 属(1933 年,刺胞动物门,粘孢子虫纲,双壳目)的粘孢子虫寄生虫从印度旁遮普邦兰吉特萨加尔湿地栖息的鱼类鳍中分离出来。疟原虫直径为 0.5 - 0.7 毫米,每个含有 80 - 100 个粘孢子。粘孢子在瓣面观呈卵形至椭圆形,前端钝尖,后端宽圆,大小为 8.36×4.77 微米。极囊单个,宽梨形,大小为 4.77×3.98 微米,含有一条垂直于粘孢子体纵轴盘绕 7 - 9 圈的极丝。对分离株 18S rDNA 序列的 Blast 分析表明,与 种(KU155561,未发表)的同源性为 98%,其次与 (KM252684)和 种 FCO(KR819273)的同源性为 91%。如鳍疟原虫指数所示(FPI = 1),感染强度记录为轻度。在本研究中,已使用 18S rRNA 基因对 进行了描述,并使用 MEGA.6 进行了系统发育分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e40/5396810/8e0230ed1f25/mbrc-6-013-g001.jpg

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