人细胞色素c基因的构建及其在酿酒酵母中的功能表达。

Construction of a human cytochrome c gene and its functional expression in Saccharomyces cerevisiae.

作者信息

Tanaka Y, Ashikari T, Shibano Y, Amachi T, Yoshizumi H, Matsubara H

机构信息

Institute for Fundamental Research, Suntory, Ltd., Osaka.

出版信息

J Biochem. 1988 Jun;103(6):954-61. doi: 10.1093/oxfordjournals.jbchem.a122393.

DOI:10.1093/oxfordjournals.jbchem.a122393

PMID:2844747

Abstract

The nucleotide sequences of a partial cDNA and three pseudogenes of human cytochrome c were determined. The complete nucleotide sequences which encode human cytochrome c were constructed on the basis of one of the pseudogenes by in vitro mutagenesis. The constructed human cytochrome c was functionally expressed in Saccharomyces cerevisiae. The recombinant human cytochrome c was purified and characterized.

摘要

测定了人细胞色素c的部分cDNA及三个假基因的核苷酸序列。基于其中一个假基因，通过体外诱变构建了编码人细胞色素c的完整核苷酸序列。构建的人细胞色素c在酿酒酵母中实现了功能性表达。对重组人细胞色素c进行了纯化和鉴定。