Dai Jiewen, Si Jiawen, Ouyang Ningjuan, Zhang Jianfei, Wu Dandan, Wang Xudong, Shen Guofang
Department of Oral and Cranio‑maxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, P.R. China.
Mol Med Rep. 2017 May;15(5):2443-2450. doi: 10.3892/mmr.2017.6315. Epub 2017 Mar 10.
Distal-less homeobox 2 (Dlx2) is a member of the homeodomain family of transcription factors and is important for the development of cranial neural crest cells (CNCCs)‑derived craniofacial tissues. Previous studies revealed that Dlx2 was expressed in the cementum and a targeted null mutation disrupted tooth development in mice. However, whether Dlx2 overexpression may impair in vivo tooth morphogenesis remains to be elucidated. The present study used a transgenic mouse model to specifically overexpress Dlx2 in neural crest cells in order to identify the dental phenotypes in mice by observation, micro‑computed tomography and histological examination. The Dlx2‑overexpressed mice exhibited tooth abnormalities including incisor cross‑bite, shortened tooth roots, increased cementum deposition, periodontal ligament disorganization and osteoporotic alveolar bone. Therefore, Dlx2 overexpression may alter the alveolar bone, cementum and periodontal ligament (PDL) phenotypes in mice.
远端缺失同源盒2（Dlx2）是转录因子同源结构域家族的成员，对颅神经嵴细胞（CNCCs）衍生的颅面组织的发育很重要。先前的研究表明，Dlx2在牙骨质中表达，靶向无效突变会破坏小鼠的牙齿发育。然而，Dlx2过表达是否会损害体内牙齿形态发生仍有待阐明。本研究使用转基因小鼠模型在神经嵴细胞中特异性过表达Dlx2，以便通过观察、微型计算机断层扫描和组织学检查来鉴定小鼠的牙齿表型。Dlx2过表达的小鼠表现出牙齿异常，包括门牙反咬合、牙根缩短、牙骨质沉积增加、牙周膜紊乱和骨质疏松性牙槽骨。因此，Dlx2过表达可能会改变小鼠的牙槽骨、牙骨质和牙周膜（PDL）表型。
