Buganza Tepole Adrian, Vaca Elbert E, Purnell Chad A, Gart Michael, McGrath Jennifer, Kuhl Ellen, Gosain Arun K
Mechanical Engineering, Purdue University;
Division of Plastic Surgery, Ann and Robert H. Lurie Children's Hospital of Chicago, Northwestern University Feinberg School of Medicine.
J Vis Exp. 2017 Apr 16(122):55052. doi: 10.3791/55052.
Tissue expansion is a popular technique in plastic and reconstructive surgery that grows skin in vivo for correction of large defects such as burns and giant congenital nevi. Despite its widespread use, planning and executing an expansion protocol is challenging due to the difficulty in measuring the deformation imposed at each inflation step and over the length of the procedure. Quantifying the deformation fields is crucial, as the distribution of stretch over time determines the rate and amount of skin grown at the end of the treatment. In this manuscript, we present a method to study tissue expansion in order to gain quantitative knowledge of the deformations induced during an expansion process. This experimental protocol incorporates multi-view stereo and isogeometric kinematic analysis in a porcine model of tissue expansion. Multi-view stereo allows three-dimensional geometric reconstruction from uncalibrated sequences of images. The isogeometric kinematic analysis uses splines to describe the regional deformations between smooth surfaces with few mesh points. Our protocol has the potential to bridge the gap between basic scientific inquiry regarding the mechanics of skin expansion and the clinical setting. Eventually, we expect that the knowledge gained with our methodology will enable treatment planning using computational simulations of skin deformation in a personalized manner.
组织扩张是整形和重建外科中一种常用的技术，它能在体内使皮肤生长，以修复大面积缺损，如烧伤和巨大先天性痣。尽管其应用广泛，但由于难以测量每次膨胀步骤以及整个手术过程中所施加的变形，制定和执行扩张方案具有挑战性。量化变形场至关重要，因为拉伸随时间的分布决定了治疗结束时皮肤生长的速率和数量。在本手稿中，我们提出一种研究组织扩张的方法，以便获得关于扩张过程中诱导变形的定量知识。该实验方案在猪组织扩张模型中结合了多视图立体视觉和等几何运动学分析。多视图立体视觉允许从未校准的图像序列进行三维几何重建。等几何运动学分析使用样条来描述具有少量网格点的光滑表面之间的区域变形。我们的方案有可能弥合关于皮肤扩张力学的基础科学研究与临床环境之间的差距。最终，我们期望通过我们的方法获得的知识将能够以个性化方式使用皮肤变形的计算模拟进行治疗规划。
