Miyasaki K T, de Camargo P M, Wolinsky L E
Section of Periodontics, School of Denistry, University of California, Los Angeles 90024.
J Dent Res. 1988 Oct;67(10):1300-6. doi: 10.1177/00220345880670101101.
In this study, the modulation of adherence of hydrogen peroxide (H2O2)-producing and non-H2O2-producing strains of oral streptococci by the host leukocyte enzyme myeloperoxidase (MPO) was examined. It was found that exposure to MPO decreased adherence of many strains of oral streptococci to saliva-coated hydroxyapatite beads in the presence of exogenous H2O2 and chloride. The MPO-H2O2-Cl-system increased the adherence of one strain. In the absence of exogenous H2O2, the MPO-H2O2-Cl-system decreased the adherence of H2O2-producing strains only. Glucose increased streptococcal H2O2 production and also increased the anti-adhesive activity of MPO in the absence of exogenous H2O2. We conclude that: (1) host leukocytes can modulate the adherence of oral streptococci via MPO; (2) endogenous production of H2O2 by the oral streptococci can provide sufficient substrate H2O2 to drive this system; and (3) MPO will exert differential modulatory effects on the adherence of oral streptococci, based in part upon the level of endogenous H2O2 production and in part upon the particular characteristics of the adhesins of the bacteria.
在本研究中，检测了宿主白细胞酶髓过氧化物酶（MPO）对产过氧化氢（H2O2）和不产H2O2的口腔链球菌菌株黏附的调节作用。结果发现，在存在外源性H2O2和氯化物的情况下，暴露于MPO会降低许多口腔链球菌菌株对唾液包被的羟基磷灰石珠的黏附。MPO-H2O2-Cl系统增加了一株菌株的黏附。在没有外源性H2O2的情况下，MPO-H2O2-Cl系统仅降低产H2O2菌株的黏附。葡萄糖增加了链球菌H2O2的产生，并且在没有外源性H2O2的情况下也增加了MPO的抗黏附活性。我们得出以下结论：（1）宿主白细胞可通过MPO调节口腔链球菌的黏附；（2）口腔链球菌内源性产生的H2O2可为该系统提供足够的底物H2O2；（3）MPO对口腔链球菌的黏附将产生不同的调节作用，部分基于内源性H2O2的产生水平，部分基于细菌黏附素的特定特性。
