Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medicine School of Ningbo University, Ningbo, Zhejiang, China.
Brief Bioinform. 2018 Sep 28;19(5):930-945. doi: 10.1093/bib/bbx042.
Long noncoding RNAs (lncRNAs) had been defined as a novel class of functional RNAs longer than 200 nucleotides around a decade ago. It is widely acknowledged that lncRNAs play a significant role in regulation of gene expression, but the biological and molecular mechanisms are diverse and complex, and remain to be determined. Especially, the regulatory network of lncRNAs associated with other biological molecules is still a controversial matter, thus becoming a new frontier of the studies on transcriptome. Recent advance in high-throughput sequencing technologies and bioinformatics approaches may be an accelerator to lift the mysterious veil. In this review, we will outline well-known associations between lncRNAs and other biological molecules, demonstrate the diverse bioinformatics approaches applied in prediction and analysis of lncRNA interaction and perform a case study for lncRNA linc00460 to concretely decipher the lncRNA regulatory network.
长链非编码 RNA（lncRNA）大约十年前被定义为一类新型的功能性 RNA，长度超过 200 个核苷酸。人们普遍认为 lncRNA 在基因表达调控中发挥重要作用，但生物和分子机制是多样化和复杂的，仍有待确定。特别是，与其他生物分子相关的 lncRNA 调控网络仍然是转录组研究的一个争议问题，因此成为一个新的前沿领域。高通量测序技术和生物信息学方法的最新进展可能是揭开这一神秘面纱的加速器。在这篇综述中，我们将概述 lncRNA 与其他生物分子之间的已知关联，展示应用于 lncRNA 相互作用预测和分析的多样化生物信息学方法，并对 lncRNA linc00460 进行案例研究，以具体解析 lncRNA 调控网络。
