Bowden R A, Dobbs S, Kopecky K J, Crawford S, Meyers J D
Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
J Infect Dis. 1988 Oct;158(4):773-9. doi: 10.1093/infdis/158.4.773.
Cytotoxic activity of bronchoalveolar lavage (BAL) cells from 23 bone marrow transplant recipients against CMV-infected fibroblast target cells was evaluated to determine if responses differed between patients with and without CMV pneumonia. Responses of BAL cells were also compared with responses of peripheral blood lymphocytes (PBLs). BAL cells from patients with CMV pneumonia had greater cytotoxicity than BAL cells from patients with non-CMV pneumonia (P = .008). Cytotoxicity of BAL cells was also increased, as compared with PBLs, from patients with CMV pneumonia (P = .008). Lymphocytes, not alveolar macrophages, were found to be the cytotoxic effector cells in studies using purified BAL populations. Depletion studies using Leu 11b and complement suggested that natural killer cells were responsible for the cytotoxic activity. These studies demonstrate that BAL cells from patients with CMV pneumonia after bone marrow transplant have increased cytotoxicity against CMV-infected target cells.
评估了23名骨髓移植受者支气管肺泡灌洗（BAL）细胞对巨细胞病毒（CMV）感染的成纤维细胞靶细胞的细胞毒性活性，以确定患有和未患有CMV肺炎的患者之间的反应是否存在差异。还将BAL细胞的反应与外周血淋巴细胞（PBL）的反应进行了比较。患有CMV肺炎的患者的BAL细胞比未患有CMV肺炎的患者的BAL细胞具有更强的细胞毒性（P = 0.008）。与PBL相比，患有CMV肺炎的患者的BAL细胞的细胞毒性也有所增加（P = 0.008）。在使用纯化的BAL群体的研究中发现，细胞毒性效应细胞是淋巴细胞，而非肺泡巨噬细胞。使用Leu 11b和补体的耗竭研究表明，自然杀伤细胞负责细胞毒性活性。这些研究表明，骨髓移植后患有CMV肺炎的患者的BAL细胞对CMV感染的靶细胞的细胞毒性增加。
