Gyllenhammar H, Hafström I, Ringertz B, Uden A M, Palmblad J
Department of Medicine 3, Karolinska Institute, Södersjukhuser, Stockholm, Sweden.
J Interferon Res. 1988 Aug;8(4):441-9. doi: 10.1089/jir.1988.8.441.
Recombinant human interferon-alpha 2 (rIFN-alpha A) was evaluated as a modulator of neutrophil functions. Neutrophils treated with rIFN-alpha A for 1 h in vitro showed reduced chemiluminescence (CL) and aggregation in response to phagocytosis. In contrast, when certain soluble stimuli [f-met-leu-phe (fMLP) or leukotriene B4] were used, rIFN-alpha A treatment conferred a doubling of CL. This was paralleled by a similar increase in superoxide anion production and a 56% increase of release of beta-glucuronidase and lysozyme. The NBT test showed that IFN treatment did not increase the number of responding neutrophils. However, there was a significant increase in the displaceable binding of fML[3H]P. Enzyme release, aggregation, and CL in response to other soluble stimuli, the ionophore A23187 and phorbol myristate acetate were unaffected by IFN treatment. Likewise, chemotaxis was not affected. Thus, phagocytosis-associated events and aggregation were hampered by rIFN-alpha A whereas secretory responses to receptor-dependent soluble stimuli were augmented. The mechanism for the latter is most likely dependent on the observed modulation of binding of fMLP to its receptor.
重组人干扰素α2（rIFN-αA）作为中性粒细胞功能的调节剂进行了评估。体外经rIFN-αA处理1小时的中性粒细胞，在吞噬作用时化学发光（CL）和聚集反应降低。相反，当使用某些可溶性刺激物[甲酰甲硫氨酸亮氨酸苯丙氨酸（fMLP）或白三烯B4]时，rIFN-αA处理使CL增加一倍。同时，超氧阴离子产生也有类似增加，β-葡萄糖醛酸酶和溶菌酶释放增加56%。NBT试验表明，干扰素处理并未增加反应性中性粒细胞的数量。然而，fML[3H]P的可置换结合显著增加。对其他可溶性刺激物、离子载体A23187和佛波酯肉豆蔻酸酯乙酸盐的酶释放、聚集和CL不受干扰素处理的影响。同样，趋化性也未受影响。因此，rIFN-αA阻碍了吞噬相关事件和聚集，而增强了对受体依赖性可溶性刺激物的分泌反应。后者的机制很可能取决于观察到的fMLP与其受体结合的调节。
